PXD004958

PXD004958 is an original dataset announced via ProteomeXchange.

Title	Proteome and peptidome of Vipera berus berus venom
Description	Snake venom is a rich source of peptides and proteins with a wide range of actions. Many of the components of the venom are currently being tested for their usefulness in the treatment of many diseases ranging from neurological and cardiovascular to cancer. It is also important to constantly search for new proteins and peptides with properties not yet described. The venom of Vipera berus berus has hemolytic, proteolytic and cytotoxic properties, but its exact composition and the factors responsible for these properties are not known. Therefore, an attempt was made to identify proteins and peptides derived from this species venom by using high resolution two-dimensional electrophoresis and MALDI ToF/ToF mass spectrometry. A total of 11 protein classes have been identified mainly proteases but also L-amino acid oxidases, C-type lectin like proteins, cysteine-rich venom proteins and phospholipases A2 and 5 peptides of molecular weight less than 1500 Da. Most of the identified proteins are responsible for the highly hemotoxic properties of the venom. Presence of venom phospholipases A2 and L- amino acid oxidases cause moderate neuro-, myo- and cytotoxicity. All successfully identified peptides belong to the bradikinin-potentiating peptides family.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_02:17:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrzej Łyskowski
SpeciesList	scientific name: Vipera berus berus; NCBI TaxID: 31156;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-13 08:37:44	ID requested
1	2016-12-12 07:45:50	announced
⏵ 2	2017-10-24 02:17:41	announced	Updated project metadata.

Bocian A, Urbanik M, Hus K, Ł, yskowski A, Petrilla V, Andrej, č, á, kov, á Z, Petrillov, á M, Legath J, Proteome and Peptidome of Vipera berus berus Venom. Molecules, 21(10):(2016) [pubmed]

curator keyword: Biological

submitter keyword: Vipera berus berus, venom, proteome, peptidome, cytotoxicity

Aleksandra Bocian
contact affiliation	Rzeszow University Technology Faculty of Chemistry - Department of Biotechnology and Bioinformatics al. Powstancow Warszawy 6 35-959 Rzeszow, Poland
contact email	bocian@prz.edu.pl
lab head
Andrzej Łyskowski
contact affiliation	Rzeszow University of Technology, Faculty of Chemistry, Rzeszow, Poland
contact email	andrzej.lyskowski@prz.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004958

PRIDE project URI

• PRIDE
  1. PXD004958
     1. Label: PRIDE project
     2. Name: Proteome and peptidome of Vipera berus berus venom