PXD004954

PXD004954 is an original dataset announced via ProteomeXchange.

Title	Proteomics and metabolomics reveal changes in cancer cells after alternation of SHMT2 expressions
Description	In order to understand the underline mechanism of SHMT2 (serine hydroxymethyltransferase 2) effect on tumor growth, proteome and metabolome analysis were carried on a engineered HeLa cell line (HeLa-SHMT2-shSHMT2, short as HeLa-Ss), which has inducible SHMT2 over-expression or suppression by treating cell with tetracycline or IPTG, respectively. SHMT2 over-expression in HeLa-ss cell increased cell proliferation in vitro and in vivo, deceased expression of several mitochondrial complex I and III proteins, and increased glycine and glutathione levels in cells. BioID method identified more than 20 SHMT2 associated proteins that are involved in oxidation-reduction process. These results indicate SHMT2 involves in the regulation of cellular redox balance. SHMT2 repression only reduced growth of cells under glycine depletion condition. It increased expression of several proteins involved in glutaminolysis and amino acid transporters, and elevated metabolites related to glutamine metabolism. These results indicate tumor cells have a compensatory reaction after SHMT2 suppression. Further reducing glycine levels in cells by sodium benzoate caused cell death in cultured cell and slightly reduced tumor growth in vivo. Benzoate treatment induces more changes in protein expressions and metabolite levels and may be a new approach to inhibit tumor growth.
HostingRepository	PRIDE
AnnounceDate	2020-08-19
AnnouncementXML	Submission_2020-08-18_22:33:39.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004954
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jonathan Krieger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Deamidated; Dehydrated; TMT6plex; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-13 02:20:04	ID requested
⏵ 1	2020-08-18 22:33:39	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: SHMT2, quantitative proteomics, interactome, metabolism, sodium benzoate, LC-MS/MS

Michael F Moran
contact affiliation	Program in Cell Biology Hospital For Sick Children, Toronto, SPARC BioCentre Hospital For Sick Children, Toronto, 8Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
contact email	m.moran@utoronto.ca
lab head
Jonathan Krieger
contact affiliation	SickKids/University of Toronto
contact email	Jonathan.krieger@sickkids.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004954
     1. Label: PRIDE project
     2. Name: Proteomics and metabolomics reveal changes in cancer cells after alternation of SHMT2 expressions