PXD004954 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics and metabolomics reveal changes in cancer cells after alternation of SHMT2 expressions |
Description | In order to understand the underline mechanism of SHMT2 (serine hydroxymethyltransferase 2) effect on tumor growth, proteome and metabolome analysis were carried on a engineered HeLa cell line (HeLa-SHMT2-shSHMT2, short as HeLa-Ss), which has inducible SHMT2 over-expression or suppression by treating cell with tetracycline or IPTG, respectively. SHMT2 over-expression in HeLa-ss cell increased cell proliferation in vitro and in vivo, deceased expression of several mitochondrial complex I and III proteins, and increased glycine and glutathione levels in cells. BioID method identified more than 20 SHMT2 associated proteins that are involved in oxidation-reduction process. These results indicate SHMT2 involves in the regulation of cellular redox balance. SHMT2 repression only reduced growth of cells under glycine depletion condition. It increased expression of several proteins involved in glutaminolysis and amino acid transporters, and elevated metabolites related to glutamine metabolism. These results indicate tumor cells have a compensatory reaction after SHMT2 suppression. Further reducing glycine levels in cells by sodium benzoate caused cell death in cultured cell and slightly reduced tumor growth in vivo. Benzoate treatment induces more changes in protein expressions and metabolite levels and may be a new approach to inhibit tumor growth. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-19 |
AnnouncementXML | Submission_2020-08-18_22:33:39.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004954 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jonathan Krieger |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Ammonia-loss; Deamidated; Dehydrated; TMT6plex; Oxidation; Acetyl; Carbamidomethyl |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-09-13 02:20:04 | ID requested | |
⏵ 1 | 2020-08-18 22:33:39 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: SHMT2, quantitative proteomics, interactome, metabolism, sodium benzoate, LC-MS/MS |
Contact List
Michael F Moran |
contact affiliation | Program in Cell Biology Hospital For Sick Children, Toronto, SPARC BioCentre Hospital For Sick Children, Toronto, 8Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada |
contact email | m.moran@utoronto.ca |
lab head | |
Jonathan Krieger |
contact affiliation | SickKids/University of Toronto |
contact email | Jonathan.krieger@sickkids.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004954
- Label: PRIDE project
- Name: Proteomics and metabolomics reveal changes in cancer cells after alternation of SHMT2 expressions