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PXD004954

PXD004954 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics and metabolomics reveal changes in cancer cells after alternation of SHMT2 expressions
DescriptionIn order to understand the underline mechanism of SHMT2 (serine hydroxymethyltransferase 2) effect on tumor growth, proteome and metabolome analysis were carried on a engineered HeLa cell line (HeLa-SHMT2-shSHMT2, short as HeLa-Ss), which has inducible SHMT2 over-expression or suppression by treating cell with tetracycline or IPTG, respectively. SHMT2 over-expression in HeLa-ss cell increased cell proliferation in vitro and in vivo, deceased expression of several mitochondrial complex I and III proteins, and increased glycine and glutathione levels in cells. BioID method identified more than 20 SHMT2 associated proteins that are involved in oxidation-reduction process. These results indicate SHMT2 involves in the regulation of cellular redox balance. SHMT2 repression only reduced growth of cells under glycine depletion condition. It increased expression of several proteins involved in glutaminolysis and amino acid transporters, and elevated metabolites related to glutamine metabolism. These results indicate tumor cells have a compensatory reaction after SHMT2 suppression. Further reducing glycine levels in cells by sodium benzoate caused cell death in cultured cell and slightly reduced tumor growth in vivo. Benzoate treatment induces more changes in protein expressions and metabolite levels and may be a new approach to inhibit tumor growth.
HostingRepositoryPRIDE
AnnounceDate2020-08-19
AnnouncementXMLSubmission_2020-08-18_22:33:39.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD004954
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJonathan Krieger
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListAmmonia-loss; Deamidated; Dehydrated; TMT6plex; Oxidation; Acetyl; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-09-13 02:20:04ID requested
12020-08-18 22:33:39announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: SHMT2, quantitative proteomics, interactome, metabolism, sodium benzoate, LC-MS/MS
Contact List
Michael F Moran
contact affiliationProgram in Cell Biology Hospital For Sick Children, Toronto, SPARC BioCentre Hospital For Sick Children, Toronto, 8Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
contact emailm.moran@utoronto.ca
lab head
Jonathan Krieger
contact affiliationSickKids/University of Toronto
contact emailJonathan.krieger@sickkids.ca
dataset submitter
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Dataset FTP location
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PRIDE project URI
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