PXD004945

PXD004945 is an original dataset announced via ProteomeXchange.

Title	Glioma proteome - Quantitative proteomics reveals fundamental regulatory differences in oncogenic HRAS and IDH1 driven astrocytoma
Description	In the present study, we applied a quantitative MS-based strategy to characterize the proteome and phosphoproteome in HRAS- or IDH1-driven glioma cells. We describe the driving roles of the MEK and PI3K signaling pathways in RAS-NHA cells, and uncover oncogenic signaling in other pathways. We highlight the interplay between the signaling cascades and show that inhibition of MEK and PI3K reverses phosphorylation signaling patterns driven by oncogenic RAS overexpression. Applying a histone hybrid chemical labeling method and high-resolution MS, we identified significant histone methylation, acetylation, and butyrylation changes in IDH1mut-NHA cells. Our results suggest a global transcriptional repressive state, consistent with the down-regulation of the proteome, transcriptome, and the DNA hyper-methylated state in IDH1mut-NHA cells. We provide a unique resource of altered proteins, phosphosites, and histone PTMs in RAS and IDH1 mutant astrocytoma cell lines, providing insight into oncogenesis in glioma beyond the transcriptomic level.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:09:56.792.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophia Doll
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; 6x(13)C labeled residue
Instrument	LTQ Orbitrap Velos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-12 05:56:46	ID requested
1	2016-12-23 11:49:29	announced
2	2017-10-24 06:13:26	announced	Updated project metadata.
⏵ 3	2024-10-22 04:09:59	announced	2024-10-22: Updated project metadata.

Doll S, Urisman A, Oses-Prieto JA, Arnott D, Burlingame AL, Quantitative Proteomics Reveals Fundamental Regulatory Differences in Oncogenic HRAS and Isocitrate Dehydrogenase (IDH1) Driven Astrocytoma. Mol Cell Proteomics, 16(1):39-56(2017) [pubmed]

curator keyword: Biological

submitter keyword: Phosphoproteomics, MEK PI3K inhibition,Primary vs. secondary Glioblastoma, Epigenetics

Sophia Doll
contact affiliation	Department of pharmaceutical chemistry, school of pharmacy, University of California San Francisco
contact email	doll@biochem.mpg.de
lab head
Sophia Doll
contact affiliation	Max Planck Institute of Biochemsitry
contact email	doll@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004945

PRIDE project URI

• PRIDE
  1. PXD004945
     1. Label: PRIDE project
     2. Name: Glioma proteome - Quantitative proteomics reveals fundamental regulatory differences in oncogenic HRAS and IDH1 driven astrocytoma