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PXD004945

PXD004945 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlioma proteome - Quantitative proteomics reveals fundamental regulatory differences in oncogenic HRAS and IDH1 driven astrocytoma
DescriptionIn the present study, we applied a quantitative MS-based strategy to characterize the proteome and phosphoproteome in HRAS- or IDH1-driven glioma cells. We describe the driving roles of the MEK and PI3K signaling pathways in RAS-NHA cells, and uncover oncogenic signaling in other pathways. We highlight the interplay between the signaling cascades and show that inhibition of MEK and PI3K reverses phosphorylation signaling patterns driven by oncogenic RAS overexpression. Applying a histone hybrid chemical labeling method and high-resolution MS, we identified significant histone methylation, acetylation, and butyrylation changes in IDH1mut-NHA cells. Our results suggest a global transcriptional repressive state, consistent with the down-regulation of the proteome, transcriptome, and the DNA hyper-methylated state in IDH1mut-NHA cells. We provide a unique resource of altered proteins, phosphosites, and histone PTMs in RAS and IDH1 mutant astrocytoma cell lines, providing insight into oncogenesis in glioma beyond the transcriptomic level.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:09:56.792.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSophia Doll
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; 6x(13)C labeled residue
InstrumentLTQ Orbitrap Velos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-09-12 05:56:46ID requested
12016-12-23 11:49:29announced
22017-10-24 06:13:26announcedUpdated project metadata.
32024-10-22 04:09:59announced2024-10-22: Updated project metadata.
Publication List
Doll S, Urisman A, Oses-Prieto JA, Arnott D, Burlingame AL, Quantitative Proteomics Reveals Fundamental Regulatory Differences in Oncogenic HRAS and Isocitrate Dehydrogenase (IDH1) Driven Astrocytoma. Mol Cell Proteomics, 16(1):39-56(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Phosphoproteomics, MEK PI3K inhibition,Primary vs. secondary Glioblastoma, Epigenetics
Contact List
Sophia Doll
contact affiliationDepartment of pharmaceutical chemistry, school of pharmacy, University of California San Francisco
contact emaildoll@biochem.mpg.de
lab head
Sophia Doll
contact affiliationMax Planck Institute of Biochemsitry
contact emaildoll@biochem.mpg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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