PXD004944

PXD004944 is an original dataset announced via ProteomeXchange.

Title	Complementary metaproteomic approaches to assess marine bacteriopankton response towards algal bloom
Description	In this project, the metaproteome of the marine bacterioplankton was analyzed to assess its respone towards an algal bloom in the southern North Sea in spring 2010. Proteins were extracted applying two different methods: (i) applying chemical cell lysis using trifluoroethanol in combination with in-solution digest and (ii) mechanical cell lysis applying bead beating, SDS-PAGE prefractionation and in-gel digest. Both samples were analyzed by nanoLC and ESI-iontrap MS. In case of the TFE lysis samples, also nanoLC-MALDI-TOF MS was applied.
HostingRepository	PRIDE
AnnounceDate	2017-04-11
AnnouncementXML	Submission_2017-04-11_07:02:35.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004944
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lars Wöhlbrand
SpeciesList	scientific name: environmental samples; NCBI TaxID: 48479;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	amaZon Speed ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-12 05:24:15	ID requested
⏵ 1	2017-04-11 07:02:37	announced

W, ö, hlbrand L, Wemheuer B, Feenders C, Ruppersberg HS, Hinrichs C, Blasius B, Daniel R, Rabus R, Complementary Metaproteomic Approaches to Assess the Bacterioplankton Response toward a Phytoplankton Spring Bloom in the Southern North Sea. Front Microbiol, 8():442(2017) [pubmed]

curator keyword: Biological

submitter keyword: metaproteome, marine bacterioplankton, algal bloom

Lars Wöhlbrand
contact affiliation	Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl-von-Ossietzky-University Oldenburg, Oldenburg, Germany
contact email	lars.woehlbrand@uni-oldenburg.de
lab head
Lars Wöhlbrand
contact affiliation	General and Molecular Microbiology
contact email	lars.woehlbrand@uni-oldenburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD004944

PRIDE project URI

• PRIDE
  1. PXD004944
     1. Label: PRIDE project
     2. Name: Complementary metaproteomic approaches to assess marine bacteriopankton response towards algal bloom