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PXD004934

PXD004934 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of novel RHOA interacting patners
DescriptionRHO subfamily of small GTPases comprise highly conserved family members RHOA, RHOB, and RHOC which cycle between GTP-bound 'active' and GDP-bound 'inactive' states. In the active form, RHO proteins interact with a variety of downstream effector proteins, controlling their activity and function. Many of the RHO subfamily effector proteins such as ROCK, PKN, and mDIA, are key regulators of actin cytoskeleton and cell motility. To identify novel effector proteins for RHOA,we carried out a GST-pulldown from heavy or light SILAC labelled HeLa cells using GST tagged GTP-bound RHOA, or GST alone control, as bait. Pulldowns were performed in duplicates with switched labellings. Specific interactors were destinguished from the background on the basis of SILAC Heavy to Light ratios between GST-RHOA and GST alone pulldowns.
HostingRepositoryPRIDE
AnnounceDate2016-11-01
AnnouncementXMLSubmission_2016-11-01_03:22:19.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFaraz Mardakheh
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-09-12 02:55:12ID requested
12016-11-01 03:22:20announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: SILAC
LC-MS
protein-protein interactions
RHOA
Contact List
Christopher J Marshall
contact affiliationDivision of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, The United Kingdom.
contact emailchris.marshall@icr.ac.uk
lab head
Faraz Mardakheh
contact affiliationInstitute of Cancer Research
contact emailMARDAKHEH@ICR.AC.UK
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
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