PXD004924

PXD004924 is an original dataset announced via ProteomeXchange.

Title	Identification of autophosphorylationsites in recombinant Saccharomyces cerevisiae Ire1
Description	This study reports autophosphorylation sites in the serine/threonine protein kinase Ire1. The cytosolic portion of Ire1 was expressed as an glutathione S transferase fusion protein in Escherichia coli. Phosphroyaltion sites in the recombinant wild type protein and a L745A mutant were mapped by mass spectrometry. Phosphorylation sites map of the activation loop in the protein kinase domain and the alphaEF-alphaF insertion loop. In the activation loop phosphorylation was observed at serine 837, serine 840, serine 844, and threonine 844. Phosphorylaton was also observed at serine 850 in the P+1 loop. In the alphaEF-alphaF insertion loop phosphorylation was observed at threonine 873, serine 877, serine 878, threonine 881, serine 884, serine 885, and serine 887.
HostingRepository	PRIDE
AnnounceDate	2017-06-09
AnnouncementXML	Submission_2017-06-08_23:56:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Schroeder
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monomethylated residue; phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-12 01:58:02	ID requested
⏵ 1	2017-06-08 23:56:07	announced

Armstrong MC, Š, estak S, Ali AA, Sagini HAM, Brown M, Baty K, Treumann A, Schr, ö, der M, Bypass of Activation Loop Phosphorylation by Aspartate 836 in Activation of the Endoribonuclease Activity of Ire1. Mol Cell Biol, 37(16):(2017) [pubmed]

curator keyword: Biological

submitter keyword: Yeast, Ire1, protein kinase, phosphorylation, unfolded protein response

Martin Schröder
contact affiliation	Durham University, Department of Biosciences, South Road, Durham DH1 3LE, United Kingdom
contact email	martin.schroeder@durham.ac.uk
lab head
Martin Schroeder
contact affiliation	Durham University
contact email	martin.schroeder@durham.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004924
     1. Label: PRIDE project
     2. Name: Identification of autophosphorylationsites in recombinant Saccharomyces cerevisiae Ire1