PXD004918

PXD004918 is an original dataset announced via ProteomeXchange.

Title	Monitoring of protein thiol-oxidation in S.aureus USA300 under hypochlorite stress using OxICAT approach.
Description	During infections, S. aureus has to cope with the oxidative burst of activated macrophages and neutrophils, including reactive oxygen and nitrogen species (RNS, ROS) and the strong oxidant hypochloric acid. We aimed to understand the global thiol-redox state in the major pathogen S. aureus and discover new NaOCl-sensitive proteins driven by thiol-switches. Thus, we performed a quantitative redox proteomics approach based on OxICAT and analyzed the percentages of thiol-oxidation levels in S.aureus before and after sub-lethal doses of 150 µM NaOCl stress. In parallel, we searched for protein S-bacillithiolation.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:10:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcel Imber
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	Applied Biosystems cleavable ICAT(TM) heavy; Applied Biosystems cleavable ICAT(TM) light
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-12 00:10:44	ID requested
1	2016-12-21 23:13:41	announced
⏵ 2	2017-10-24 03:10:49	announced	Updated project metadata.

Imber M, Huyen NTT, Pietrzyk-Brzezinska AJ, Loi VV, Hillion M, Bernhardt J, Th, ä, richen L, Kol, š, ek K, Saleh M, Hamilton CJ, Adrian L, Gr, ä, ter F, Wahl MC, Antelmann H, Protein S-Bacillithiolation Functions in Thiol Protection and Redox Regulation of the Glyceraldehyde-3-Phosphate Dehydrogenase Gap in Staphylococcus aureus Under Hypochlorite Stress. Antioxid Redox Signal, 28(6):410-430(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Staphylococcus aureus, OxICAT, oxidative stress, GAPDH, NaOCl, H2O2

Haike Antelmann
contact affiliation	FU Berlin Institut für biologie-mikrobiologie Germany
contact email	haike.antelmann@fu-berlin.de
lab head
Marcel Imber
contact affiliation	FU Berlin, EMA Greifswald
contact email	marcel.imber@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004918

PRIDE project URI

• PRIDE
  1. PXD004918
     1. Label: PRIDE project
     2. Name: Monitoring of protein thiol-oxidation in S.aureus USA300 under hypochlorite stress using OxICAT approach.