PXD004917

PXD004917 is an original dataset announced via ProteomeXchange.

Title	Charting Organellar Importomes by Quantitative Mass Spectrometry - ATOM40p
Description	Protein import into organelles is essential for all eukaryotes and facilitated by multi-protein translocation machineries. Analyzing whether a protein is transported into an organelle is largely restricted to single constituents. This renders knowledge about imported proteins incomplete, limiting our understanding of organellar biogenesis and function. Here, we introduce a method that enables to chart an organelle’s importome. Our method relies on inducible RNAi-mediated knockdown of an essential subunit of a translocase to impair import, metabolic labeling and quantitative mass spectrometry. To highlight its potential, we established the mitochondrial importome of Trypanosoma brucei, comprising 1,120 proteins including 331 new candidates. An exciting feature of this method is that it overcomes limitations in identifying proteins with dual or multiple locations. We demonstrate the specificity and versatility of this novel ImportOmics method by targeting import machineries in mitochondria and glycosomes demonstrating its high potential for globally studying protein import and inventories of organelles.
HostingRepository	PRIDE
AnnounceDate	2017-06-14
AnnouncementXML	Submission_2017-06-14_05:18:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Friedel Drepper
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-12 00:05:03	ID requested
⏵ 1	2017-06-14 05:18:16	announced

Peikert CD, Mani J, Morgenstern M, K, ä, ser S, Knapp B, Wenger C, Harsman A, Oeljeklaus S, Schneider A, Warscheid B, Charting organellar importomes by quantitative mass spectrometry. Nat Commun, 8():15272(2017) [pubmed]

curator keyword: Biological

submitter keyword: SILAC, RNAi-mediated protein knockdown, proteomics, Trypanosoma brucei, organellar protein import, mitochondria, glycosomes, ImportOmics

Prof. Dr. Bettina Warscheid
contact affiliation	Department of Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology and BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg im Breisgau, Germany
contact email	Bettina.Warscheid@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliation	AG Warscheid Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg Germany
contact email	friedel.drepper@biologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD004917

PRIDE project URI

• PRIDE
  1. PXD004917
     1. Label: PRIDE project
     2. Name: Charting Organellar Importomes by Quantitative Mass Spectrometry - ATOM40p