PXD004906

PXD004906 is an original dataset announced via ProteomeXchange.

Title	Multisite phosphorylation of NuMA-related LIN-5 controls mitotic spindle positioning in C. elegans
Description	Chromosome segregation and cleavage plane determination involves the generation of pulling forces on microtubules that extend from the centrosomes to the cell cortex. These forces depend on cortical anchoring of the cytoplasmic dynein motor by a conserved ternary complex of Galpha, GPR-1/2, and LIN-5 proteins in C. elegans (Galpha-LGN-NuMA in mammals). Previously, we showed that the polarity kinase PKC-3 phosphorylates LIN-5 to control spindle positioning in early C. elegans embryos. Here, we investigate whether additional LIN-5 phosphorylations regulate cortical pulling forces, making use of targeted alteration of in vivo phosphorylated residues by CRISPR/Cas9-mediated genetic engineering. Four distinct in vivo phosphorylated LIN-5 residues were found to have critical functions in spindle positioning. Two of these residues form part of a 30 amino acid binding site for GPR-1, which we identified by reverse two-hybrid screening. We provide evidence for a dualkinase mechanism, involving GSK3 phosphorylation of S659 followed by phosphorylation of S662 by casein kinase 1. These LIN-5 phosphorylations promote LIN-5-GPR-1/2 interaction and contribute to cortical pulling forces. The other two critical residues, T168 and T181, form part of a cyclin-dependent kinase consensus site and are phosphorylated by CDK1-Cyclin B in vitro. We applied a novel strategy to characterize early embryonic defects in lethal T168/T181 knockin substitution mutants, and provide evidence for sequential LIN-5 N-terminal phosphorylation and dephosphorylation in dynein recruitment. Our data support that phosphorylation of multiple LIN-5 domains by different kinases contributes to a mechanism for spatiotemporal control of spindle positioning and chromosome segregation.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_14:00:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Javier Munoz
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-09 05:46:50	ID requested
⏵ 1	2018-10-26 14:00:43	announced

Portegijs V, Fielmich LE, Galli M, Schmidt R, Mu, ñ, oz J, van Mourik T, Akhmanova A, Heck AJ, Boxem M, van den Heuvel S, Multisite Phosphorylation of NuMA-Related LIN-5 Controls Mitotic Spindle Positioning in C. elegans. PLoS Genet, 12(10):e1006291(2016) [pubmed]

curator keyword: Biological

submitter keyword: phosphorylation, LIN5, spindle

Sander van den Heuvel
contact affiliation	Professor of Developmental Biology Director Institute of Biodynamics and Biocomplexity
contact email	S.J.L.vandenHeuvel@uu.nl
lab head
Javier Munoz
contact affiliation	CNIO
contact email	jmunozpe@cnio.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004906
     1. Label: PRIDE project
     2. Name: Multisite phosphorylation of NuMA-related LIN-5 controls mitotic spindle positioning in C. elegans