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PXD004904

PXD004904 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
DescriptionTo increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showing the superior sensitivity of the Hp-RP StageTip approach.
HostingRepositoryjPOST
AnnounceDate2016-09-08
AnnouncementXMLSubmission_2022-09-18_03:40:58.597.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPei-Yi Lin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-09-07 23:59:47ID requested
12016-09-07 23:59:49announced
22016-11-17 18:22:40announcedUpdated publication list.
32018-09-18 23:23:28announcedUpdated CV, FTP location and Modification list.
42021-02-04 17:07:07announced2021-02-04: Updated FTP location.
52022-09-18 03:40:59announced2022-09-18: Updated FTP location.
Publication List
Dimayacyac-Esleta BR, Tsai CF, Kitata RB, Lin PY, Choong WK, Lin TD, Wang YT, Weng SH, Yang PC, Arco SD, Sung TY, Chen YJ, Rapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling. Anal Chem, 87(24):12016-23(2015) [pubmed]
Keyword List
submitter keyword: Membrane Proteomic, Rapid High-pH Reverse Phase StageTip
Contact List
Baby Rorielyn T. Dimayacyac-Esleta and Yu-Ju Chen
lab head
Pei-Yi Lin
contact affiliationAcademia Sinica
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000184/