PXD004893

PXD004893 is an original dataset announced via ProteomeXchange.

Title	Increased cysteine protease activity in the aphid midgut decreases the transmission efficiency of a circulative plant virus
Description	Aphids, sap-sucking insects in the order Hemiptera, are among the most prolific insect vectors of plant viruses Plant viruses from the family Luteoviridae are transmitted exclusively by aphids in a circulative manner and cause significant crop yield losses. Circulative plant viruses must cross the aphid gut and other tissues prior to transmission to a new host plant. The discovery of proteins that control acquisition and transmission in the insect vector is the biggest challenge for the vector biology field and will have practical applications for growers by providing new molecular targets for the development of precision vector management tools. The green peach aphid, Myzus persicae, is a vector of the Potato leafroll virus (PLRV), a polerovirus in the Luteoviridae. PLRV transmission efficiency was significantly reduced when a clonal lineage of M. persicae was reared on turnip (T-Myzus) as compared to the weed physalis (P-Myzus). The effect on PLRV transmission efficiency was transient and caused by a host-switch response. Using 2-D DIGE, we revealed that the major difference in the proteome profile of P- and T-Myzus was the lysosomal cysteine protease cathepsin B, with multiple size and charge isoforms of this enzyme up-regulated in T-Myzus. Quantitative, shotgun proteomics revealed a specific upregulation in the expression of other lysosomal proteins in T-Myzus as compared to P-Myzus, including cathepsin B, cathepsin B-16, beta-glucuronidase, peroxidasin, legumain-like, and aminopeptidase-N. The titer of PLRV was over 1.5 fold higher in P-Myzus than in T-Myzus at 24h and 72h after the beginning of virus acquisition, suggesting that virus acquisition in P-Myzus was more efficient. Cathepsin B and PLRV localization were starkly different in P- and T-Myzus midguts, the site of PLRV acquisition into the insect. In P-Myzus midguts, an abundance of PLRV was observed inside midgut cells, and cathepsin B was sequestered in a subcellular compartment. In contrast, there is near complete co-localization of cathepsin B and PLRV at the cell membranes in viruliferous T-Myzus. Inhibition of cathepsin and other cysteine proteases with E64 restored the ability of T-Myzus to transmit PLRV in a dose-dependent manner, suggesting that the activities of lysosomal cysteine proteases at the cell membrane in T-Myzus is responsible for the change in virus transmission phenotype in these aphids. T-Myzus individuals weighed more and had more progeny than P-Myzus individuals. These data are all consistent with the hypothesis that there is an induction of lysosomal exocytos in the midgut of T-Myzus linked to the ability of the aphid to acquire PLRV. These data also show that the ability of the generalist aphid M. persicae to transmit PLRV is influenced by the host plant the aphids are reared on, information that is useful to growers for polerovirus management in field crops.
HostingRepository	PRIDE
AnnounceDate	2017-01-17
AnnouncementXML	Submission_2017-01-17_07:35:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004893
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kevin Howe
SpeciesList	scientific name: Myzus persicae persicae; NCBI TaxID: 1087439;
ModificationList	Methylthio; Deamidated; Oxidation
Instrument	TSQ Vantage; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-24 06:26:41	ID requested
1	2017-01-02 13:00:06	announced
⏵ 2	2017-01-17 07:35:21	announced	Updated project metadata.

Pinheiro PV, Ghanim M, Alexander M, Rebelo AR, Santos RS, Orsburn BC, Gray S, Cilia M, Host Plants Indirectly Influence Plant Virus Transmission by Altering Gut Cysteine Protease Activity of Aphid Vectors. Mol Cell Proteomics, 16(4 suppl 1):S230-S243(2017) [pubmed]

curator keyword: Biological

submitter keyword: Luteoviridae, Potato leafroll virus, host-pathogen interactions, 2D-DIGE, LC-MSMS

Michelle Cilia
contact affiliation	US Department of Agriculture, Agricultural Research Service (USDA-ARS), Boyce Thompson Institute, Cornell University
contact email	mlc68@cornell.edu
lab head
Kevin Howe
contact affiliation	USDA-ARS
contact email	kjh46@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004893
     1. Label: PRIDE project
     2. Name: Increased cysteine protease activity in the aphid midgut decreases the transmission efficiency of a circulative plant virus