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PXD004890

PXD004890 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of ablation of mitochondrial fission in adult forebrain neurons
DescriptionWell-balanced mitochondrial fission and fusion processes are essential for nervous system development. Loss of function of the main mitochondrial fission mediator, dynamin-related protein 1 (Drp1), is lethal early during embryonic development or around birth, but the role of mitochondrial fission in adult neurons remains unclear. Here we show that inducible Drp1 ablation in neurons of the adult mouse forebrain results in progressive, neuronal subtype-specific alterations of mitochondrial morphology in the hippocampus that are marginally responsive to antioxidant treatment. Furthermore, DRP1 loss affects synaptic transmission and memory function. Although these changes culminate in hippocampal atrophy, they are not sufficient to cause neuronal cell death within 10 weeks of genetic Drp1 ablation. Collectively, our in vivo observations clarify the role of mitochondrial fission in neurons, demonstrating that Drp1 ablation in adult forebrain neurons compromises critical neuronal functions without causing overt neurodegeneration.
HostingRepositoryPRIDE
AnnounceDate2017-01-17
AnnouncementXMLSubmission_2017-01-17_07:34:26.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD004890
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexander Schmidt
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListOxidation; Oxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-08-23 06:58:38ID requested
12016-08-25 08:59:46announced
22017-01-17 07:34:27announcedUpdated project metadata.
Publication List
Oettinghaus B, Schulz JM, Restelli LM, Licci M, Savoia C, Schmidt A, Schmitt K, Grimm A, Morè L, Hench J, Tolnay M, Eckert A, D'Adamo P, Franken P, Ishihara N, Mihara K, Bischofberger J, Scorrano L, Frank S, Synaptic dysfunction, memory deficits and hippocampal atrophy due to ablation of mitochondrial fission in adult forebrain neurons. Cell Death Differ, 23(1):18-28(2016) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: TMT, LC-MS/MS, neurons, synaptic dysfunction
Contact List
Alexander Schmidt
contact affiliationBiozentrum, University of Basel, Klinglebergstrasse 50/70, CH-4056 Basel
contact emailalex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliationProteomics Core Facility
contact emailalex.schmidt@unibas.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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