PXD004890 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of ablation of mitochondrial fission in adult forebrain neurons |
Description | Well-balanced mitochondrial fission and fusion processes are essential for nervous system development. Loss of function of the main mitochondrial fission mediator, dynamin-related protein 1 (Drp1), is lethal early during embryonic development or around birth, but the role of mitochondrial fission in adult neurons remains unclear. Here we show that inducible Drp1 ablation in neurons of the adult mouse forebrain results in progressive, neuronal subtype-specific alterations of mitochondrial morphology in the hippocampus that are marginally responsive to antioxidant treatment. Furthermore, DRP1 loss affects synaptic transmission and memory function. Although these changes culminate in hippocampal atrophy, they are not sufficient to cause neuronal cell death within 10 weeks of genetic Drp1 ablation. Collectively, our in vivo observations clarify the role of mitochondrial fission in neurons, demonstrating that Drp1 ablation in adult forebrain neurons compromises critical neuronal functions without causing overt neurodegeneration. |
HostingRepository | PRIDE |
AnnounceDate | 2017-01-17 |
AnnouncementXML | Submission_2017-01-17_07:34:26.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004890 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alexander Schmidt |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | Oxidation; Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-08-23 06:58:38 | ID requested | |
1 | 2016-08-25 08:59:46 | announced | |
⏵ 2 | 2017-01-17 07:34:27 | announced | Updated project metadata. |
Publication List
Oettinghaus B, Schulz JM, Restelli LM, Licci M, Savoia C, Schmidt A, Schmitt K, Grimm A, Mor, รจ L, Hench J, Tolnay M, Eckert A, D'Adamo P, Franken P, Ishihara N, Mihara K, Bischofberger J, Scorrano L, Frank S, Synaptic dysfunction, memory deficits and hippocampal atrophy due to ablation of mitochondrial fission in adult forebrain neurons. Cell Death Differ, 23(1):18-28(2016) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: TMT, LC-MS/MS, neurons, synaptic dysfunction |
Contact List
Alexander Schmidt |
contact affiliation | Biozentrum, University of Basel, Klinglebergstrasse 50/70, CH-4056 Basel |
contact email | alex.schmidt@unibas.ch |
lab head | |
Alexander Schmidt |
contact affiliation | Proteomics Core Facility |
contact email | alex.schmidt@unibas.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004890
- Label: PRIDE project
- Name: Analysis of ablation of mitochondrial fission in adult forebrain neurons