PXD004882

PXD004882 is an original dataset announced via ProteomeXchange.

Title	Greatwall kinase regulates SAC by controlling phosphorylation and activity of MPS1
Description	The Greatwall/Mastl kinase is an essential gene, which regulates the phosphatase activity that counteracts the phosphorylation of Cdk1 substrates. Although Mastl-/- MEFs can enter mitosis, they are unable to complete mitosis due to chromosome segregation defects. Here, we show that Mastl is essential for robust spindle assembly checkpoint (SAC) maintenance. Mastl-/- MEFs display reduced phosphorylation and kinase activity of MPS1, which causes mislocalization of MPS1, Mad1, and Aurora B from the kinetochore and the inner centromere regions. This results in premature inactivation of SAC signalling and early anaphase onset without correction of chromosome-spindle attachment mistakes. Treatment of the knockout cells with protein phosphatase II inhibitor okadaic acid (OA) rescued decreased MPS1 activity, mislocalization of Aurora B, Mad1, MPS1, and premature mitotic slippage. Our data demonstrates how regulation of PP2A activity by Mastl kinase prevents premature SAC silencing as a result of controlling the phosphorylation status and activity of MPS1.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_14:03:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sheena Wee
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-22 04:04:44	ID requested
⏵ 1	2018-10-26 14:03:20	announced

Diril MK, Bisteau X, Kitagawa M, Caldez MJ, Wee S, Gunaratne J, Lee SH, Kaldis P, Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint. PLoS Genet, 12(9):e1006310(2016) [pubmed]

curator keyword: Biological

submitter keyword: Greatwall kinase/Mastl/cyclin-dependent kinase/knockout mice/Cdk1/MPS1/SAC

Jayantha Gunaratne
contact affiliation	Institute of Molecular and Cell Biology (IMCB), A*STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Proteos#3-09, Singapore 138673, Republic of Singapore
contact email	jayanthag@imcb.a-star.edu.sg
lab head
Sheena Wee
contact affiliation	IMCB
contact email	swee@imcb.a-star.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004882

PRIDE project URI

• PRIDE
  1. PXD004882
     1. Label: PRIDE project
     2. Name: Greatwall kinase regulates SAC by controlling phosphorylation and activity of MPS1