PXD004879

PXD004879 is an original dataset announced via ProteomeXchange.

Title	Subcellular proteomics analysis of different stages of colorectal cancer cell lines
Description	Studying cell differentiation and transformation allows a better understanding of the mechanisms involved in the initiation and the evolution of cancer. The role of proteins which participate in these processes is dependent on their location within the cell. Determining the subcellular localization of proteins or the changes in localization is, therefore, paramount in elucidating their role. Using quantitative mass spectrometry, we characterized the protein expression and subcellular localization of nearly 5,000 proteins from seven different colorectal cancer (CRC) cell lines, as well as normal colon fibroblasts and intestinal epithelial cells. This cellular characterization allowed the identification of colon cancer-associated proteins with differential expression patterns as well as deregulated protein networks and pathways. Indeed, our results demonstrate differential expression of proteins involved in cell adhesion, cytoskeleton, and transcription in colon cancer cells compared to normal colon-derived cells. Pathway analyses identified different cellular functions, including endocytosis and eIF2 signaling, whose deregulation correlates with mutations found in the different CRC phenotypes. Our results provide an unbiased, quantitative and high-throughput approach to measure changes in protein expression and subcellular protein locations in different colorectal cancer cell lines.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:09.130.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francois-Michel Boisvert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-22 03:09:59	ID requested
1	2017-04-19 02:06:33	announced
⏵ 2	2024-10-22 04:32:15	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201600314;

Mathieu AA, Ohl-S, é, guy E, Dubois ML, Jean D, Jones C, Boudreau F, Boisvert FM, Subcellular proteomics analysis of different stages of colorectal cancer cell lines. Proteomics, 16(23):3009-3018(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: subcellular proteomics,Colorectal cancer, SILAC, colon

Francois-Michel Boisvert
contact affiliation	Department of Anatomy and Cell Biology, Université de Sherbrooke
contact email	fm.boisvert@usherbrooke.ca
lab head
Francois-Michel Boisvert
contact affiliation	Université de Sherbrooke
contact email	fm.boisvert@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD004879

PRIDE project URI

• PRIDE
  1. PXD004879
     1. Label: PRIDE project
     2. Name: Subcellular proteomics analysis of different stages of colorectal cancer cell lines