PXD004876

PXD004876 is an original dataset announced via ProteomeXchange.

Title	Characterization of genetic loss-of-function of Fus in zebrafish
Description	The RNA-binding protein FUS is implicated in transcription, alternative splicing of neuronal genes and DNA repair. Mutations in FUS have been linked to human neurodegenerative diseases such as ALS (amyotrophic lateral sclerosis). We genetically disrupted fus in zebrafish (Danio rerio) using the CRISPR-Cas9 system. The fus knockout animals are fertile and did not show any distinctive phenotype. Mutation of fus induces mild changes in gene expression on the transcriptome and proteome level in the adult brain. We observed a significant influence of genetic background on gene expression and 3’UTR usage, which could mask the effects of loss of Fus. Unlike published fus morphants, maternal zygotic fus mutants do not show motoneuronal degeneration and exhibit normal locomotor activity.
HostingRepository	PRIDE
AnnounceDate	2018-06-15
AnnouncementXML	Submission_2018-06-15_05:44:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-19 05:47:57	ID requested
⏵ 1	2018-06-15 05:44:59	announced

Lebedeva S, de Jesus Domingues AM, Butter F, Ketting RF, Characterization of genetic loss-of-function of Fus in zebrafish. RNA Biol, 14(1):29-35(2017) [pubmed]

curator keyword: Biological

submitter keyword: zebrafish, fus protein, CRISPR

Falk Butter
contact affiliation	Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
lab head
F Butter
contact affiliation	Dept. of Proteomics and Signal Transduction
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD004876

PRIDE project URI

• PRIDE
  1. PXD004876
     1. Label: PRIDE project
     2. Name: Characterization of genetic loss-of-function of Fus in zebrafish