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PXD004868

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-08-11
  • AnnouncementXML: Submission_2017-08-11_04:03:53.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Harsha Gowda
  • Title: Altered signaling associated with chronic arsenic exposure in human skin keratinocytes
  • Description: Modulation of signaling pathways upon chronic arsenic exposure remains poorly studied. Here we carried out SILAC-based quantitative phosphoproteomics analysis to dissect the signaling induced upon chronic arsenic exposure in human skin keratinocyte cell line, HaCaT. We identified 4,171 unique phosphosites derived from 2,000 proteins. We observed differential phosphorylation of 406 phosphosites (2-fold) corresponding to 305 proteins. Several pathways involved in cytoskeleton maintenance and organization were found to be significantly enriched (p<0.05). Our data revealed altered phosphorylation of proteins associated with adherens junction remodeling and actin polymerization. Kinases such as protein kinase C iota type (PRKCI), mitogen-activated protein kinase kinase kinase 1 (MAP3K1), tyrosine-protein kinase BAZ1B (BAZ1B) and STE20 like kinase (SLK) were found to be hyperphosphorylated. Our study provides novel insights into signaling perturbations associated with chronic arsenic exposure in human skin keratinocytes.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: phosphorylated residue; 6x(13)C labeled residue; monohydroxylated residue
  • Instrument: LTQ Orbitrap Velos

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-08-19 01:47:50ID requested
12017-08-11 04:03:55announced

Publication List

  1. Mir SA, Renuse S, Sathe G, Khan AA, Patil AH, Nanjappa V, Bhat FA, Prasad TSK, Giri AK, Chatterjee A, Gowda H, Altered signaling associated with chronic arsenic exposure in human skin keratinocytes. Proteomics Clin Appl, ():(2017) [pubmed]

Keyword List

  1. submitter keyword: HaCaT, SILAC, Arsenic, Chronic exposure

Contact List

    Harsha Gowda
    • contact affiliation: Institute of Bioinformatics, Bangalore, India
    • contact email: harsha@ibioinformatics.org
    • lab head:
    Harsha Gowda
    • contact affiliation: Institute of Bioinformatics
    • contact email: harsha@ibioinformatics.org
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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