PXD004862

PXD004862 is an original dataset announced via ProteomeXchange.

Title	Quantification of sulfenic acid modification in an atherosclerosis
Description	Physiological stimuli such as thrombin, or pathological stimuli such as lysophosphatidic acid (LPA), activate platelets. The activated platelets bind to monocytes through P-selectin-PSGL-1 interactions, but also release the contents of their granules, commonly called “platelet releasate”. It is known that monocytes in contact with platelet releasate produce reactive oxygen species (ROS). Reversible cysteine oxidation by ROS is considered to be a potential regulator of protein function. In a previous study, we used THP-1 monocytic cells exposed to LPA- or thrombin-induced platelet releasate and a modified biotin switch assay to unravel the biological processes that are influenced by reversible cysteine oxidation. To gain a better understanding of the redox regulation of monocytes in atherosclerosis, we have now altered the modified biotin switch to selectively quantify protein sulfenic acid, a subpopulation of reversible cysteine oxidation. Using arsenite as reducing agent in the modified biotin switch assay, we were able to quantify 1161 proteins, in which more than 100 sulfenic acid sites were identified. Bioinformatics analysis of the quantified sulfenic acid sites highlighted the relevant, previously missed biological process of monocyte transendothelial migration, which included integrin β2. Flow cytometry validated the activation of LFA-1 (αLβ2) and Mac-1 (αMβ2), two subfamilies of integrin β2 complexes, on human primary monocytes following platelet releasate treatment. The activation of LFA-1 was mediated by ROS from NADPH oxidase (NOX) activation. Production of ROS and activation of LFA-1 in human primary monocytes were independent of P-selectin-PSGL-1 interaction. Our results proved the modified biotin switch assay to be a powerful tool with the ability to reveal new regulatory mechanisms and identify new therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:06.907.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ru Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-18 01:47:02	ID requested
1	2016-10-10 03:24:38	announced
⏵ 2	2024-10-22 04:32:09	announced	2024-10-22: Updated project metadata.

Li R, Klockenbusch C, Lin L, Jiang H, Lin S, Kast J, on Monocytes via NADPH Oxidase. J Proteome Res, 15(12):4221-4233(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: sulfenic acid, biotin switch assay, THP-1 cell, NOX

Juergen Kast
contact affiliation	Biomedical research centre University of British Columbia
contact email	kast@brc.ubc.ca
lab head
Ru Li
contact affiliation	Univerisity of British Columbia
contact email	rli@brc.ubc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004862
     1. Label: PRIDE project
     2. Name: Quantification of sulfenic acid modification in an atherosclerosis