Updated FTP location. The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 largeT and smallT antigens (SV40 ER). TIG3 TSM and TSR cells were transformed lines by cMyc or H-RAS(G12V) in TERT/SV40 expressing TIG-3, respectively.