Updated CV, FTP location and Modification list. The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 largeT and smallT antigens (SV40 ER). TIG3 TSM and TSR cells were transformed lines by cMyc or H-RAS(G12V) in TERT/SV40 expressing TIG-3, respectively.