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PXD004820

PXD004820 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa
DescriptionRecently, molecular hydrogen (H2) has emerged as a bio-regulator both in animals and plants. Normally, functions of endogenous generated H2 could be mimicked by exogenously applied hydrogen-rich water (HRW) or hydrogen-rich saline (particularly in animals). Although alfalfa seedlings showed more cadmium (Cd) resistance after the administration with HRW, corresponding molecular mechanism is still elusive. To address this gap, iTRAQ-based quantitative proteomics was used. In our experimental conditions, out of 238 significant differential proteins identified in the Cd- and HRW/Cd-treated samples, 60 were unique to Cd-stressed alone samples, 107 were unique to HRW plus Cd treatment samples, and 71 were shared between two groups. Furthermore, 58 proteins from the 238 proteins significantly responded in Cd- and HRW/Cd-treatment samples were selected for further bioinformatics analysis. Interestingly, results indicated that they were classified into six categories: oxidation-reduction process, carbon metabolism, citrate cycle, cysteine and methionine metabolism, metal ion homeostasis, and sulfur compound metabolic process. In addition, the protein expression patterns were consistent with the results of increased non-protein thiols abundant, as well as iron and zinc content, in seedling roots. These suggest that HRW alleviates Cd toxicity mainly by decreasing oxidative damage, enhancing sulfur compound metabolic process, and maintaining nutrient element homeostasis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:32:21.211.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChen Dai
SpeciesList scientific name: Medicago sativa (Alfalfa); NCBI TaxID: 3879;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-08-16 05:11:28ID requested
12017-01-04 08:00:47announced
22017-10-24 04:02:28announcedUpdated project metadata.
32024-10-22 04:32:29announced2024-10-22: Updated project metadata.
Publication List
Dai C, Cui W, Pan J, Xie Y, Wang J, Shen W, Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa. J Proteomics, 152():109-120(2017) [pubmed]
10.1016/j.jprot.2016.10.013;
Keyword List
curator keyword: Biological
submitter keyword: Cadmium
Molecular hydrogen
iTRAQ
Medicago sativa
Contact List
Weiti Cui and Chen Dai
contact affiliationCollege of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China
contact emaildaichencpu@outlook.com
lab head
Chen Dai
contact affiliationNanjing Agricultural University
contact emaildaichencpu@outlook.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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