PXD004820

PXD004820 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa
Description	Recently, molecular hydrogen (H2) has emerged as a bio-regulator both in animals and plants. Normally, functions of endogenous generated H2 could be mimicked by exogenously applied hydrogen-rich water (HRW) or hydrogen-rich saline (particularly in animals). Although alfalfa seedlings showed more cadmium (Cd) resistance after the administration with HRW, corresponding molecular mechanism is still elusive. To address this gap, iTRAQ-based quantitative proteomics was used. In our experimental conditions, out of 238 significant differential proteins identified in the Cd- and HRW/Cd-treated samples, 60 were unique to Cd-stressed alone samples, 107 were unique to HRW plus Cd treatment samples, and 71 were shared between two groups. Furthermore, 58 proteins from the 238 proteins significantly responded in Cd- and HRW/Cd-treatment samples were selected for further bioinformatics analysis. Interestingly, results indicated that they were classified into six categories: oxidation-reduction process, carbon metabolism, citrate cycle, cysteine and methionine metabolism, metal ion homeostasis, and sulfur compound metabolic process. In addition, the protein expression patterns were consistent with the results of increased non-protein thiols abundant, as well as iron and zinc content, in seedling roots. These suggest that HRW alleviates Cd toxicity mainly by decreasing oxidative damage, enhancing sulfur compound metabolic process, and maintaining nutrient element homeostasis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:21.211.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chen Dai
SpeciesList	scientific name: Medicago sativa (Alfalfa); NCBI TaxID: 3879;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-16 05:11:28	ID requested
1	2017-01-04 08:00:47	announced
2	2017-10-24 04:02:28	announced	Updated project metadata.
⏵ 3	2024-10-22 04:32:29	announced	2024-10-22: Updated project metadata.

Dai C, Cui W, Pan J, Xie Y, Wang J, Shen W, Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa. J Proteomics, 152():109-120(2017) [pubmed]

10.1016/j.jprot.2016.10.013;

curator keyword: Biological

submitter keyword: Cadmium

Molecular hydrogen

iTRAQ

Medicago sativa

Weiti Cui and Chen Dai
contact affiliation	College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China
contact email	daichencpu@outlook.com
lab head
Chen Dai
contact affiliation	Nanjing Agricultural University
contact email	daichencpu@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004820
     1. Label: PRIDE project
     2. Name: Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa