PXD004820 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa |
Description | Recently, molecular hydrogen (H2) has emerged as a bio-regulator both in animals and plants. Normally, functions of endogenous generated H2 could be mimicked by exogenously applied hydrogen-rich water (HRW) or hydrogen-rich saline (particularly in animals). Although alfalfa seedlings showed more cadmium (Cd) resistance after the administration with HRW, corresponding molecular mechanism is still elusive. To address this gap, iTRAQ-based quantitative proteomics was used. In our experimental conditions, out of 238 significant differential proteins identified in the Cd- and HRW/Cd-treated samples, 60 were unique to Cd-stressed alone samples, 107 were unique to HRW plus Cd treatment samples, and 71 were shared between two groups. Furthermore, 58 proteins from the 238 proteins significantly responded in Cd- and HRW/Cd-treatment samples were selected for further bioinformatics analysis. Interestingly, results indicated that they were classified into six categories: oxidation-reduction process, carbon metabolism, citrate cycle, cysteine and methionine metabolism, metal ion homeostasis, and sulfur compound metabolic process. In addition, the protein expression patterns were consistent with the results of increased non-protein thiols abundant, as well as iron and zinc content, in seedling roots. These suggest that HRW alleviates Cd toxicity mainly by decreasing oxidative damage, enhancing sulfur compound metabolic process, and maintaining nutrient element homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:32:21.211.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chen Dai |
SpeciesList | scientific name: Medicago sativa (Alfalfa); NCBI TaxID: 3879; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-08-16 05:11:28 | ID requested | |
1 | 2017-01-04 08:00:47 | announced | |
2 | 2017-10-24 04:02:28 | announced | Updated project metadata. |
⏵ 3 | 2024-10-22 04:32:29 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dai C, Cui W, Pan J, Xie Y, Wang J, Shen W, Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa. J Proteomics, 152():109-120(2017) [pubmed] |
10.1016/j.jprot.2016.10.013; |
Keyword List
curator keyword: Biological |
submitter keyword: Cadmium |
Molecular hydrogen |
iTRAQ |
Medicago sativa |
Contact List
Weiti Cui and Chen Dai |
contact affiliation | College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China |
contact email | daichencpu@outlook.com |
lab head | |
Chen Dai |
contact affiliation | Nanjing Agricultural University |
contact email | daichencpu@outlook.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004820
- Label: PRIDE project
- Name: Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa