PXD004818

PXD004818 is an original dataset announced via ProteomeXchange.

Title	Spectral histopathology supported laser capture microdissection and quantitative proteome analysis of diffuse malignant mesothelioma
Description	FTIR based spectral histopathology was used for label-free classification of tumor tissue sections. The regions of interest were identified and annotated with high spatial resolution. The spatial information was used for automated laser capture microdissection of relevant tissue areas. Subsequently, the collected tissue samples were analyzed using quantitative proteomics. This novel approach for precise sample collection in clinical studies was applied to differentiate epitheloid and sarcomatoid subtypes of diffuse malignant mesothelioma.
HostingRepository	PRIDE
AnnounceDate	2017-03-31
AnnouncementXML	Submission_2017-03-31_04:27:30.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004818
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thilo Bracht
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	residues isobaric at 128.058578 Da; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-16 01:21:59	ID requested
⏵ 1	2017-03-31 04:27:31	announced

Gro, ß, erueschkamp F, Bracht T, Diehl HC, Kuepper C, Ahrens M, Kallenbach-Thieltges A, Mosig A, Eisenacher M, Marcus K, Behrens T, Br, ü, ning T, Theegarten D, Sitek B, Gerwert K, Spatial and molecular resolution of diffuse malignant mesothelioma heterogeneity by integrating label-free FTIR imaging, laser capture microdissection and proteomics. Sci Rep, 7():44829(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Colorectal carcinoma, diffuse malignant mesothelioma, laser capture microdissection, infrared spectral histopathology, FTIR

Barbara Sitek
contact affiliation	Ruhr-Universität Bochum Medizinisches Proteom-Center AG Clinical Proteomics
contact email	barbara.sitek@rub.de
lab head
Thilo Bracht
contact affiliation	Clinical Proteomics
contact email	thilo.bracht@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD004818

PRIDE project URI

• PRIDE
  1. PXD004818
     1. Label: PRIDE project
     2. Name: Spectral histopathology supported laser capture microdissection and quantitative proteome analysis of diffuse malignant mesothelioma