PXD004814

PXD004814 is an original dataset announced via ProteomeXchange.

Title	PLRV co-immunoprecipitation from Solanum tuberosum
Description	Phloem localization of plant viruses is advantageous for acquisition by sap-sucking vectors but hampers host-virus protein interaction studies. In this study, Potato leafroll virus (PLRV)-host protein complexes were isolated from systemically infected potato, a natural host of the virus. Comparing two different co-immunoprecipitation support matrices coupled to mass spectrometry, we identified 44 potato proteins and one viral protein (P1) specifically associated with virus isolated from infected phloem. An additional 142 proteins interact in complex with virus at varying degrees of confidence. Greater than 80% of these proteins were previously found to form high confidence interactions with PLRV isolated from the model host Nicotiana benthamiana. Bioinformatics revealed that these proteins are enriched for functions related to plasmodesmata, organelle membrane transport, translation and mRNA processing. Our results show that model system proteomics experiments are extremely valuable for understanding protein interactions regulating infection in recalcitrant pathogens such as phloem-limited viruses.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_02:56:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stacy DeBlasio
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-15 09:02:17	ID requested
1	2016-12-20 20:06:45	announced
⏵ 2	2017-10-24 02:56:09	announced	Updated project metadata.

DeBlasio SL, Johnson RS, MacCoss MJ, Gray SM, Cilia M, Model System-Guided Protein Interaction Mapping for Virus Isolated from Phloem Tissue. J Proteome Res, 15(12):4601-4611(2016) [pubmed]

curator keyword: Biological

submitter keyword: Luteoviridae, polerovirus, Potato leafroll virus, host-pathogen interactions, phloem-limited, co-immunoprecipitation, LC-MSMS, Solanum tuberosum

Michelle Cilia
contact affiliation	United States Department of Agriculture-Agricultural Research Service (USDA-ARS); Boyce Thompson Institute; Cornell University
contact email	mlc68@cornell.edu
lab head
Stacy DeBlasio
contact affiliation	USDA-ARS
contact email	sld98@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004814

PRIDE project URI

• PRIDE
  1. PXD004814
     1. Label: PRIDE project
     2. Name: PLRV co-immunoprecipitation from Solanum tuberosum