PXD004801

PXD004801 is an original dataset announced via ProteomeXchange.

Title	Improved plasma membrane proteome coverage with a label-free non-affinity-purified workflow
Description	The proteins of the cellular plasma membrane perform important functions relating to homeostasis and intercellular communication. Due to its overall low cellular abundance, amphipathic character, and low membrane-to-cytoplasm ratio, the plasma membrane proteome has been challenging to isolate and characterize, and is poorly represented in standard liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. In this study, we employ a label-free, non-affinity-purified workflow for the enrichment of the plasma membrane proteome and use subsequent bioinformatics tools to select plasma membrane proteins, herein referred to as the surfaceome. Using this methodology, we identified over 2500 cell surface-associated proteins in a human acute myeloid leukemia (AML) cell line. These surface proteins comprised 60% of all detected cellular proteins, a number that substantially exceeds the depth of coverage in previously published studies describing the leukemia surfaceome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:13.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Seeholzer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-15 03:00:09	ID requested
1	2017-01-31 05:12:20	announced
⏵ 2	2024-10-22 04:32:14	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201600344;

Glisovic-Aplenc T, Gill S, Spruce LA, Smith IR, Fazelinia H, Shestova O, Ding H, Tasian SK, Aplenc R, Seeholzer SH, Improved surfaceome coverage with a label-free nonaffinity-purified workflow. Proteomics, 17(7):(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: label-free

proteomics

plasma membrane

GO term

leukemia

Steven H. Seeholzer
contact affiliation	The Children's Hospital of Philadelphia Proteomics Core Facility Philadelphia, PA
contact email	seeholzer@email.chop.edu
lab head
Steven Seeholzer
contact affiliation	Children's Hospital of Philadelphia
contact email	seeholzer@email.chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD004801

PRIDE project URI

• PRIDE
  1. PXD004801
     1. Label: PRIDE project
     2. Name: Improved plasma membrane proteome coverage with a label-free non-affinity-purified workflow