PXD004800 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of the yeast peptidome and comparison with the human peptidome |
Description | Peptides function as signaling molecules in species as diverse as humans and yeast. Mass spectrometry-based peptidomics techniques provide a relatively unbiased method to assess the peptidome of biological samples. In the present study, we used a quantitative peptidomic technique to characterize the peptidome of the yeast Saccharomyces cerevisiae and compare it to the peptidomes of mammalian cell lines and tissues. Altogether, 297 yeast peptides derived from 75 proteins were identified. The yeast peptides are similar to those of the human peptidome in average size and amino acid composition. Inhibition of proteasome activity with either bortezomib or epoxomicin led to decreased levels of some yeast peptides, suggesting that these peptides are generated by the proteasome. Approximately 30% of the yeast peptides correspond to the N- or C-terminus of the protein; the human peptidome is also highly represented in N- or C-terminal protein fragments. Most yeast and humans peptides are derived from a subset of abundant proteins, many with functions involving cellular metabolism or protein synthesis and folding. Of the 75 yeast proteins that give rise to peptides, 24 have orthologs that give rise to human and/or mouse peptides and for some, the same region of the proteins are found in the human, mouse, and yeast peptidomes. Taken together, these results support the hypothesis that intracellular peptides may have specific and conserved biological functions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:06:37.139.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexandre Keiji Tashima |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; acetylated residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-08-15 02:58:04 | ID requested | |
1 | 2020-06-02 00:41:50 | announced | |
⏵ 2 | 2024-10-22 05:06:45 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1371/journal.pone.0163312; |
Dasgupta S, Yang C, Castro LM, Tashima AK, Ferro ES, Moir RD, Willis IM, Fricker LD, Analysis of the Yeast Peptidome and Comparison with the Human Peptidome. PLoS One, 11(9):e0163312(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: mass spectrometry, Saccharomyces cerevisiae, proteasome,Peptidome |
Contact List
Lloyd D. Fricker |
contact affiliation | Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA |
contact email | lloyd.fricker@einstein.yu.edu |
lab head | |
Alexandre Keiji Tashima |
contact affiliation | Universidade Federal de Sao Paulo |
contact email | aktashima@unifesp.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004800
- Label: PRIDE project
- Name: Analysis of the yeast peptidome and comparison with the human peptidome