PXD004781

PXD004781 is an original dataset announced via ProteomeXchange.

Title	Multiplexed temporal quantification of the human plasma peptidome
Description	The plasma peptidome contains the entire complement of low molecular weight endogenous peptides derived from secretion, protease activity and post-translational modifications and is a rich source of biomarkers and novel bioactives. To examine the utility of peptidomics we have examined the effects of exercise on the peptidome because exercise is thought to convey many of its physiological benefits via the blood stream. Deep peptidomic analysis was achieved using 2D LC-MS/MS with multiple fragmentation methods and included identification of post-translational modifications and multiplexed temporal quantification. We show the plasma peptidome is subject to transient changes during exercise on a time-scale of minutes that is rapidly reversible during recovery. Known peptide hormones were acutely regulated including insulin, glucagon, ghrelin, bradykinin, cholecystokinin, secretogranins and many others. Acute regulation of proteases and post-translational modifications including glycosylation were observed. These data reveal the power of peptidomic quantification to temporally quantify bioactive peptides, biomarkers and proteolytic activity during pathophysiological processes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:18.386.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Parker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; amidated residue; monohydroxylated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-12 01:49:45	ID requested
1	2017-11-21 07:23:47	announced
⏵ 2	2024-10-22 04:32:19	announced	2024-10-22: Updated project metadata.

Parker BL, Burchfield JG, Clayton D, Geddes TA, Payne RJ, Kiens B, Wojtaszewski JFP, Richter EA, James DE, Multiplexed Temporal Quantification of the Exercise-regulated Plasma Peptidome. Mol Cell Proteomics, 16(12):2055-2068(2017) [pubmed]

10.1074/mcp.ra117.000020;

curator keyword: Biological, Biomedical

submitter keyword: peptidomics, exercise, peptidome,plasma

Benjamin Parker
contact affiliation	The University of Sydney
contact email	benjamin.parker@sydney.edua.au
lab head
Benjamin Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD004781

PRIDE project URI

• PRIDE
  1. PXD004781
     1. Label: PRIDE project
     2. Name: Multiplexed temporal quantification of the human plasma peptidome