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PXD004781

PXD004781 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMultiplexed temporal quantification of the human plasma peptidome
DescriptionThe plasma peptidome contains the entire complement of low molecular weight endogenous peptides derived from secretion, protease activity and post-translational modifications and is a rich source of biomarkers and novel bioactives. To examine the utility of peptidomics we have examined the effects of exercise on the peptidome because exercise is thought to convey many of its physiological benefits via the blood stream. Deep peptidomic analysis was achieved using 2D LC-MS/MS with multiple fragmentation methods and included identification of post-translational modifications and multiplexed temporal quantification. We show the plasma peptidome is subject to transient changes during exercise on a time-scale of minutes that is rapidly reversible during recovery. Known peptide hormones were acutely regulated including insulin, glucagon, ghrelin, bradykinin, cholecystokinin, secretogranins and many others. Acute regulation of proteases and post-translational modifications including glycosylation were observed. These data reveal the power of peptidomic quantification to temporally quantify bioactive peptides, biomarkers and proteolytic activity during pathophysiological processes.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:32:18.386.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBenjamin Parker
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; amidated residue; monohydroxylated residue
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-08-12 01:49:45ID requested
12017-11-21 07:23:47announced
22024-10-22 04:32:19announced2024-10-22: Updated project metadata.
Publication List
Parker BL, Burchfield JG, Clayton D, Geddes TA, Payne RJ, Kiens B, Wojtaszewski JFP, Richter EA, James DE, Multiplexed Temporal Quantification of the Exercise-regulated Plasma Peptidome. Mol Cell Proteomics, 16(12):2055-2068(2017) [pubmed]
10.1074/mcp.ra117.000020;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: peptidomics, exercise, peptidome,plasma
Contact List
Benjamin Parker
contact affiliationThe University of Sydney
contact emailbenjamin.parker@sydney.edua.au
lab head
Benjamin Parker
contact affiliationThe University of Melbourne
contact emailben.parker@unimelb.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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