PXD004780

PXD004780 is an original dataset announced via ProteomeXchange.

Title	The ‘PepSAVI-MS’ pipeline for natural product bioactive peptide discovery
Description	The recent increase in extensively drug resistant bacterial pathogens and the associated increase of morbidity and mortality demonstrate the immediate need for new antibiotic backbones with novel mechanisms of action. Here, we report the development of the PepSAVI-MS pipeline for bioactive peptide discovery. This highly versatile platform employs bioinformatics, statistics, and mass spectrometry to identify bioactive peptide targets from complex biological samples. We validate the use of this platform through the successful identification of known bioactive peptides from a botanical species, Viola odorata. Using this pipeline, we have widened the known antimicrobial spectrum for V. odorata cyclotides, including antibacterial activity of cycloviolacin O2 against E. faecium and A. baumannii. We further demonstrate the broad applicability of the platform through the identification of novel anticancer activities for cycloviolacins by their cytotoxicity against ovarian, breast and prostate cancer cell lines.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_04:14:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christine Kirkpatrick
SpeciesList	scientific name: Viola odorata; NCBI TaxID: 97441;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-12 01:33:01	ID requested
1	2017-01-02 11:00:33	announced
⏵ 2	2017-10-24 04:14:55	announced	Updated project metadata.

Kirkpatrick CL, Broberg CA, McCool EN, Lee WJ, Chao A, McConnell EW, Pritchard DA, Hebert M, Fleeman R, Adams J, Jamil A, Madera L, Str, ö, mstedt AA, G, ö, ransson U, Liu Y, Hoskin DW, Shaw LN, Hicks LM, The "PepSAVI-MS" Pipeline for Natural Product Bioactive Peptide Discovery. Anal Chem, 89(2):1194-1201(2017) [pubmed]

curator keyword: Biological

submitter keyword: Antimicrobial peptides, antimicrobial resistance, mass spectrometry, screening platform, anticancer, antifungal

Leslie M. Hicks
contact affiliation	Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA
contact email	lmhicks@email.unc.edu
lab head
Christine Kirkpatrick
contact affiliation	UNC Chapel Hill
contact email	ckirkpat@live.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD004780

PRIDE project URI

• PRIDE
  1. PXD004780
     1. Label: PRIDE project
     2. Name: The ‘PepSAVI-MS’ pipeline for natural product bioactive peptide discovery