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PXD004762

PXD004762 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of substrates of the CRL4-HBx E3 ubiquitin ligase
DescriptionHepatitis B Virus (HBV) remains a major public health problem, and is a major cause liver cancer worldwide. HBV infection in vivo requires the function of the HBx protein, which facilitates expression of viral genes from the episomal genome by an unknown mechanism. Evidence suggests that HBx functions as a targeting subunit for the CRL4 E3 ubiquitin ligase, redirecting the complex to target and degrade an unknown host restriction factor. We used substrate trapping proteomics to search for ubiquitylation substrates of HBx. We used MLN4924 to block CRL activity and stabilize interactions between HBx and its substrates. We then performed APMS to identify bound proteins and potential substrates.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:32:07.411.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristopher Murphy
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-08-11 06:09:01ID requested
12021-03-04 12:15:55announced
22024-10-22 04:32:15announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.celrep.2016.08.026;
Murphy CM, Xu Y, Li F, Nio K, Reszka-Blanco N, Li X, Wu Y, Yu Y, Xiong Y, Su L, Hepatitis B Virus X Protein Promotes Degradation of SMC5/6 to Enhance HBV Replication. Cell Rep, 16(11):2846-2854(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: ubiquitylation, HBV, CRL4, Cullin, substrate,HBx, MLN4924
Contact List
Lishan Su
contact affiliationDepartment of Microbiology and Immunology Lineberger Cancer Center University of North Carolina, Chapel Hill
contact emaillishan_su@med.unc.edu
lab head
Christopher Murphy
contact affiliationUNC
contact emailchrism83@hotmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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