PXD004760

PXD004760 is an original dataset announced via ProteomeXchange.

Title	Gcn4-dependent translation reduction in long-lived ribosomal protein deletion strains
Description	Deletion of several ribosomal proteins genes (RPKOs) has been shown to extend the lifespan of Saccharomyces cerevisiae in a Gcn4-dependent manner. To characterize the underlying mechanisms, we systematically analyzed the gene expression of both short- and long-lived RPKO strains at multiple levels. We found that up-regulation of amino acid biosynthesis and global down-regulation of protein synthesis are hallmarks of long-lived strains. We provide direct evidence that gene expression changes observed in long-lived strains result from translational up-regulation of GCN4 mRNA via skipping of upstream open reading frames (uORFs), in turn due to slow/defective ribosome assembly. We further demonstrate that Gcn4 acts as a transcriptional repressor on promoters of translation-related genes, thereby globally reducing protein synthesis. Our data suggest that the Gcn4-dependent increase in lifespan can be attributed partially to its ability to dampen the translation capacity of the cell, thereby engaging a well known mechanism of longevity.
HostingRepository	PRIDE
AnnounceDate	2017-07-06
AnnouncementXML	Submission_2017-07-06_05:24:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004760
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	Oxidation; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-11 05:54:23	ID requested
⏵ 1	2017-07-06 05:24:35	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: yeast, TMT, GNC4, aging

Alexander Schmidt
contact affiliation	Biozentrum, Univesity of Basel, CH-4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004760
     1. Label: PRIDE project
     2. Name: Gcn4-dependent translation reduction in long-lived ribosomal protein deletion strains