PXD004752

PXD004752 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based HepaRG Membrane Proteomics
Description	HepaRG cell, a stabilized bipotent liver progenitor cell line, exhibits hepatocyte functions only after differentiation. However, the mechanism of transition from non-differentiated to differentiated states, accompanied by proliferation and migration, is poorly understood. Little information exists for proteins involved in this process, particularly those residing in the plasma membrane. In this study, the plasma membrane protein expression change of HepaRG cell before and after differentiation were systematically analyzed using an iTRAQ labeled quantitative membrane proteomics approach. A total of 70 membrane proteins were identified to be differentially expressed. Function and disease clustering analysis showed that 11 of these proteins are involved in migration. Two key factors (MMP-14 and OCLN) were validated by qRT-PCR and Western Blot. Blockade of MMP-14, an extracellular matrix metalloprotease, by monoclonal antibody in a wound healing assay further demonstrated the importance of this protein in tumor cell migration. Even further, the MMP-14 expression correlation with HCC is confirmed by HCC cell lines and tissue samples.
HostingRepository	PRIDE
AnnounceDate	2017-02-08
AnnouncementXML	Submission_2017-02-08_06:28:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Wei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-10 07:11:43	ID requested
⏵ 1	2017-02-08 06:28:54	announced

Zhao M, Xu F, Wu F, Yu D, Su N, Zhang Y, Cheng L, Xu P, iTRAQ-Based Membrane Proteomics Reveals Plasma Membrane Proteins Change During HepaRG Cell Differentiation. J Proteome Res, 15(12):4245-4257(2016) [pubmed]

curator keyword: Biological

submitter keyword: HepaRG, membrane protein, iTRAQ

Ping Xu
contact affiliation	State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Beijing Institute of Radiation Medicine, Beijing 102206, P. R. China
contact email	xuping_bprc@126.com
lab head
Wei Wei
contact affiliation	BPRC
contact email	powerweiwei@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD004752

PRIDE project URI

• PRIDE
  1. PXD004752
     1. Label: PRIDE project
     2. Name: iTRAQ-based HepaRG Membrane Proteomics