PXD004739

PXD004739 is an original dataset announced via ProteomeXchange.

Title	Identification of Direct Upstream Kinases by Fluorescence Complementation Mass Spectrometry
Description	Kinase-substrate networks are the main components of many signal transduction pathways. Although proteome-wide studies have been successful in elucidating many important biological events including the cataloging of thousands of sites of protein phosphorylation, there is a lack of a universal method to identify the direct upstream kinases responsible for many of these modifications. We have introduced Fluorescence ComplementatiKinase-substrate networks are the main components of many signal transduction pathways. Although proteome-wide studies have been successful in elucidating many important biological events including the cataloging of thousands of sites of protein phosphorylation, there is a lack of a universal method to identify the direct upstream kinases responsible for many of these modifications. We have introduced Fluorescence Complementation Mass Spectrometry (FCMS) as the first proteomic approach that identifies direct upstream kinases in living cells by stabilizing and capturing the kinase-substrate pairs. Using FCMS, we have identified both known and novel direct kinases of cAMP response element-binding protein (CREB). on Mass Spectrometry (FCMS) as the first proteomic approach that identifies direct upstream kinases in living cells by stabilizing and capturing the kinase-substrate pairs. Using FCMS, we have identified both known and novel direct kinases of cAMP response element-binding protein (CREB).
HostingRepository	PRIDE
AnnounceDate	2017-11-08
AnnouncementXML	Submission_2017-11-08_00:06:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Justine Arrington
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-09 02:21:37	ID requested
⏵ 1	2017-11-08 00:06:09	announced

Zeng L, Wang WH, Arrington J, Shao G, Geahlen RL, Hu CD, Tao WA, Identification of Upstream Kinases by Fluorescence Complementation Mass Spectrometry. ACS Cent Sci, 3(10):1078-1085(2017) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Fluorescence complementation, FCMS, Phosphorylation, Kinases

Weiguo Andy Tao
contact affiliation	Department of Biochemistry, Purdue University, West Lafayette, IN USA
contact email	taow@purdue.edu
lab head
Justine Arrington
contact affiliation	Purdue University
contact email	jvarrington@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD004739

PRIDE project URI

• PRIDE
  1. PXD004739
     1. Label: PRIDE project
     2. Name: Identification of Direct Upstream Kinases by Fluorescence Complementation Mass Spectrometry