PXD004736

PXD004736 is an original dataset announced via ProteomeXchange.

Title	Biotinylation by antibody recognition - A novel method for proximity labeling
Description	Identification of protein-protein interactions is a major goal of biological research. Despite technical advances over the last two decades, important but still largely unsolved challenges include the high-throughput detection of interactions directly from primary tissue and the identification of interactors of insoluble proteins that form higher-order structures. We have developed a novel, proximity-based labeling approach that uses antibodies to guide biotin deposition onto adjacent proteins in fixed cells and primary tissues. We showed our method to be specific and sensitive by labeling a mitochondrial matrix protein. Next, we used this method to profile the dynamic interactome of lamin A/C in multiple cell and tissue types under various treatment conditions. Our results suggest a considerable variation in the composition of the nuclear envelope of different tissues. Of note, DNA damage response proteins Ku70 and Ku80 are more abundant in the vicinity of lamin A/C after thermal stress. The ability to detect proximal proteins and putative interactors in intact tissues, and to compare affinities quantitatively under different conditions or in the presence of disease mutations, can provide a new window into cell biology and disease pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2017-10-17
AnnouncementXML	Submission_2018-01-02_02:45:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Bar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-08 09:54:18	ID requested
1	2016-08-22 12:30:35	announced
⏵ 2	2018-01-02 02:45:17	announced	Updated publication reference for PubMed record(s): 29256494.

Bar DZ, Atkatsh K, Tavarez U, Erdos MR, Gruenbaum Y, Collins FS, Biotinylation by antibody recognition-a method for proximity labeling. Nat Methods, 15(2):127-133(2018) [pubmed]

curator keyword: Technical

submitter keyword: LMNA biotin proximiy labeling

Francis Collins
contact affiliation	NHGRI/NIH
contact email	collinsf@mail.nih.gov
lab head
Daniel Bar
contact affiliation	NHGRI
contact email	daniel.bar@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD004736

PRIDE project URI

• PRIDE
  1. PXD004736
     1. Label: PRIDE project
     2. Name: Biotinylation by antibody recognition - A novel method for proximity labeling