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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-08-11
  • AnnouncementXML: Submission_2017-08-11_00:30:21.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD004734
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Guillaume Jacquemet
  • Title: Identification of novel SHARPIN binders
  • Description: Sharpin, a multifunctional adaptor protein with oncogenic properties, regulates several signalling pathways. For example, Sharpin enhances signal-induced NF-κB signalling as part of the linear ubiquitin assembly complex (LUBAC) and inhibits integrins, the T cell receptor, caspase1 and PTEN. However, despite recent insights into Sharpin and LUBAC function, a systematic approach to identify signalling pathways regulated by Sharpin has not been reported. Here, we present the first ‘Sharpin interactome’, which identifies a large amount of novel potential Sharpin interactors. These data suggest that Sharpin and LUBAC might regulate a larger number of biological processes than previously identified, such as endosomal trafficking, RNA processing, metabolism and cytoskeleton regulation. Importantly, using the Sharpin interactome we have identified a novel role for Sharpin in lamellipodium formation. We demonstrate that Sharpin interacts with the Arp2/3 complex, a protein complex that catalyses actin filament branching, and that Sharpin and Arp2/3 colocalize in lamellipodia. We identified the Arp2/3-binding site in Sharpin and demonstrate using a specific Arp2/3-binding deficient mutant that the Sharpin-Arp2/3 interaction promotes lamellipodia formation in a LUBAC-independent fashion.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-08-08 09:15:28ID requested
12017-08-11 00:30:22announced

Publication List

  1. Khan MH, Salomaa SI, Jacquemet G, Butt U, Miihkinen M, Deguchi T, Kremneva E, Lappalainen P, Humphries MJ, Pouwels J, The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex. J Cell Sci, 130(18):3094-3107(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: SHARPIN, GFP pull-down, human

Contact List

    Jeroen Pouwels
    • contact affiliation: Turku Centre for Biotechnology, University of Turku, Turku, Finland,
    • contact email: jpouwels@btk.fi
    • lab head:
    Guillaume Jacquemet
    • contact affiliation: University of Turku
    • contact email: guillaume.jacquemet@btk.fi
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
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