PXD004733

PXD004733 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of cAMP signaling of adenylate cyclase toxin in mouse dendritic cells
Description	The whooping cough agent, Bordetella pertussis, subverts dendritic cell (DCs) functions through powerful immunomodulatory activities of its toxins. Here we focused on the signaling action of the adenylate cyclase toxin (CyaA) that targets myeloid cells expressing the αMβ2 integrin CD11b/CD18 (known as complement receptor 3 (CR3) or Mac-1). CyaA delivers an extremely catalytically potent adenylyl cyclase enzyme domain into the cytosol of phagocytes and disrupts their innate and adaptive immune functions through unregulated production of the key signaling molecule cAMP. Here we describe the global phosphoproteomic analysis of cAMP signaling in murine bone marrow-derived DCs exposed to CyaA. Gathered data reveal toxin-triggered alternations of phosphorylation status of proteins regulating actin cytoskeleton, chromatin remodeling, mTOR activity and IL-10 gene expression. The reported findings highlight the complexity of subversive physiological manipulation that is exerted on myeloid phagocytes by the cAMP-generating adenylate cyclase toxin of Bordetellae.
HostingRepository	PRIDE
AnnounceDate	2017-11-29
AnnouncementXML	Submission_2017-12-11_03:57:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivo Fabrik
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C labeled L-lysine; phosphorylated residue; monohydroxylated residue; N-acetylated residue; iodoacetamide derivatized residue; 6x(13)C labeled L-arginine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-08 09:02:52	ID requested
1	2017-11-29 01:07:36	announced
⏵ 2	2017-12-11 03:57:01	announced	Updated publication reference for PubMed record(s): 29176673.

Nov, á, k J, Fabrik I, Linhartov, á I, Link M, Č, ern, ý O, Stul, í, k J, Š, ebo P, Phosphoproteomics of cAMP signaling of Bordetella adenylate cyclase toxin in mouse dendritic cells. Sci Rep, 7(1):16298(2017) [pubmed]

curator keyword: Biological

submitter keyword: Bone marrow-derived dendritic cells, Adenylate cyclase toxin, Bordetella pertussis, Phosphoproteome

Peter Šebo
contact affiliation	Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Videnska 1083, 142 20 Prague, Czech Republic
contact email	sebo@biomed.cas.cz
lab head
Ivo Fabrik
contact affiliation	Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence
contact email	ivo.fabrik@unob.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD004733

PRIDE project URI

• PRIDE
  1. PXD004733
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of cAMP signaling of adenylate cyclase toxin in mouse dendritic cells