PXD004730

PXD004730 is an original dataset announced via ProteomeXchange.

Title	Proteomics identification of CsrA-regulated proteins in Legionella pneumophila
Description	The carbon storage regulator protein CsrA regulates cellular processes post-transcriptionally by binding to target-RNAs altering translation efficiency and/or their stability. In this dataset we identified proteins regulated by CsrA in the human bacterial pathogen Legionella pneumophila by labelfree shotgun proteomics. WT and ∆csrA bacteria were grown to exponential phase in three biological replicates. Bacteria were lysed by sonication and extracted proteins were reducted, alkylated and digested with trypsin. The resulting peptide mixtures were analyzed by LC-MS/MS and proteins were identified by the MaxQuant software. In total of 1,448 proteins were quantified by the MaxLFQ algorithm in a labelfree approach. Cluster analysis showed that expression of almost all proteins was affected by the mutation of CsrA as about half of the identified proteins were up and half were down regulated. About 15% of the proteome (238 proteins) was strongly affected with 132 proteins significantly up and 109 significantly down regulated dependent on CsrA.
HostingRepository	PRIDE
AnnounceDate	2017-09-13
AnnouncementXML	Submission_2017-09-13_03:37:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Impens Francis
SpeciesList	scientific name: Legionella pneumophila str. Paris; NCBI TaxID: 297246;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-08 06:39:48	ID requested
1	2016-11-01 03:34:48	announced
2	2017-09-13 03:34:40	announced	Updated publication reference for PubMed record(s): 28212376.
⏵ 3	2017-09-13 03:37:23	announced	Updated project metadata.

Sahr T, Rusniok C, Impens F, Oliva G, Sismeiro O, Copp, é, e JY, Buchrieser C, The Legionella pneumophila genome evolved to accommodate multiple regulatory mechanisms controlled by the CsrA-system. PLoS Genet, 13(2):e1006629(2017) [pubmed]

curator keyword: Biological

submitter keyword: Legionella pneumophila, CsrA, labelfree shotgun proteomics

Carmen Buchrieser
contact affiliation	Biology Of Intracellular Bacteria Unit, Institut Pasteur, Paris, France
contact email	cbuch@pasteur.fr
lab head
Impens Francis
contact affiliation	VIB Proteomics Expertise Center
contact email	francis.impens@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD004730

PRIDE project URI

• PRIDE
  1. PXD004730
     1. Label: PRIDE project
     2. Name: Proteomics identification of CsrA-regulated proteins in Legionella pneumophila