PXD004726

PXD004726 is an original dataset announced via ProteomeXchange.

Title	Dependence of Synaptic Protein Degradation on Proteasomal Function -Part2
Description	Synaptic function crucially depends on uninterrupted synthesis and degradation of synaptic proteins. While much has been learned on synaptic protein synthesis, little is known on the routes by which synaptic proteins are degraded. Here we systematically studied how inhibition of the ubiquitin-proteasomal system (UPS) affects the degradation rates of thousands of neuronal and synaptic proteins. We identified a number of proteins, including several proteins related to glutamate receptor trafficking, whose degradation rates were significantly slowed by UPS inhibition. Unexpectedly, however, degradation rates of most synaptic proteins were not significantly affected. Interestingly, many of the differential effects of UPS inhibition were readily explained by a quantitative framework that considered known metabolic turnover rates for the same proteins. In contrast to the limited effects on protein degradation, UPS inhibition profoundly and preferentially suppressed the synthesis of a large number of synaptic proteins. Our findings point to the importance of the UPS in the degradation of certain synaptic proteins, yet indicate that under basal conditions most synaptic proteins might be degraded through alternative pathways.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_14:52:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-08 06:02:31	ID requested
⏵ 1	2018-10-26 14:52:07	announced

Hakim V, Cohen LD, Zuchman R, Ziv T, Ziv NE, The effects of proteasomal inhibition on synaptic proteostasis. EMBO J, 35(20):2238-2262(2016) [pubmed]

curator keyword: Biological

submitter keyword: proteomics, Synaptic Protein, Degradation

Noam Ziv
contact affiliation	Technion Faculty of Medicine and Network Biology Research Laboratories,
contact email	noamz@netvision.net.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@tx.technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004726
     1. Label: PRIDE project
     2. Name: Dependence of Synaptic Protein Degradation on Proteasomal Function -Part2