PXD004722

PXD004722 is an original dataset announced via ProteomeXchange.

Title	Incomplete cytosolic iron-sulfur cluster assembly machinery of Giardia intestinalis is partially associated with mitosomes
Description	Iron - sul fur (Fe -S) clusters are essential cofactors that enable proteins to transport electrons , sense signals or catalyze chemical reactions . The maturation of dozens of Fe - S proteins in various compartments of every eukaryotic cell is driven by several assembly pathways. The ubiquitous cytosolic Fe - S cluster assembly (CIA) pathway, typically composed of eight highly conserved proteins, depends on mitochondrial Fe -S cluster assembly (ISC) machinery . Giardia intestinalis contains one of the smallest eukaryotic genomes and the mitosome, an extremely reduced mitochondrion . Because the only pathway known to be retained within this organelle is the synthesis of Fe -S clusters mediated by ISC machinery, a likely function of the mitosome is to cooperate with the CIA pathway. We investigated the cellular localization of CIA components in G. intestinalis and the origin and distribution of CIA -related components and Tah18 -like proteins in other Metamonada. We show that ortholog s of Tah18 and Dre2 are missing in these eukaryotes. In Giardia, all CIA components are exclusively cytosolic , with the important exception of Cia2 and two Nbp35 paralogs, which are also present in the mitosomes. We propose that the dual localization of Cia2 and Nbp35 proteins in Giardia might represent a novel connection between the ISC and the CIA Pathways.
HostingRepository	PRIDE
AnnounceDate	2016-09-29
AnnouncementXML	Submission_2016-09-29_05:02:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Giardia intestinalis assemblage A; NCBI TaxID: 941442;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-08 03:35:13	ID requested
⏵ 1	2016-09-29 05:02:04	announced

Pyrih J, Pyrihov, á E, Kol, í, sko M, Stojanovov, á D, Basu S, Harant K, Haindrich AC, Dole, ž, al P, Luke, š J, Roger A, Tachezy J, Minimal cytosolic iron-sulfur cluster assembly machinery of Giardia intestinalis is partially associated with mitosomes. Mol Microbiol, 102(4):701-714(2016) [pubmed]

curator keyword: Biological

submitter keyword: LC MS, Giardi Intestinalis ,

Karel Harant
contact affiliation	Laboratory of Mass Spectrometry BIOCEV Faculty of Science Charles University in Prague Průmyslová 595, 252 42 Vestec, Tel +420325873925 http://www.biocev.eu/corefacilit/omics/
contact email	harant@natur.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004722
     1. Label: PRIDE project
     2. Name: Incomplete cytosolic iron-sulfur cluster assembly machinery of Giardia intestinalis is partially associated with mitosomes