PXD004714

PXD004714 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Targeted proteome analysis of the central carbon metabolism related enzymes in S. cerevisiae mutant strains
Description	Expression profiles of 110 the central metabolism-related enzymes were obtained by the selected reaction monitoring (SRM) assay methods using LC-MS/MS from the wild type (BY4742), a GCR2 gene deletion strain, and 29 single-gene deletion strains lacking enzyme genes responsible for central carbon metabolism (including CIT1, ENO1, FBP1, GCR2, GND1, GPD1, GPM2, HOR2, HXK1, HXK2, IDH1, IDH2, IDP1, LPD1, MAE1, MDH1, MDH2, PDA1, PDC1, PFK1, PYC2, RPE1, TAL1, TDH1, TDH2, TDH3, TKL1, TPS1, TPS2, and ZWF1 genes). The central carbon metabolism is strictly controlled by modulation of enzyme expressions to maintain an essential system of living organisms. In this study, metabolic safety mechanisms in the model organism, Saccharomyces cerevisiae, were investigated by direct determination of enzyme expression levels. Targeted proteome analysis of 31 S. cerevisiae wild type and mutant strains revealed that at least 30% of the observed variations in enzyme expression levels could be explained by global regulatory mechanisms. Co-expression analysis revealed that expression levels of enzymes involved in trehalose metabolism and glycolysis changed in a coordinated manner under the control of the transcription factors for global regulation.
HostingRepository	jPOST
AnnounceDate	2017-03-22
AnnouncementXML	Submission_2022-09-18_02:20:25.525.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fumio Matsuda
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-03 19:40:42	ID requested
1	2017-03-21 16:42:07	announced
2	2018-09-18 22:57:37	announced	Updated CV, FTP location and Modification list.
3	2021-02-04 16:57:38	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 02:20:25	announced	2022-09-18: Updated FTP location.

Publication List

Keyword List

submitter keyword: Targeted proteome analysis, yeast, central carbon metabolism

submitter keyword: Targeted proteome analysis, yeast, central carbon metabolism

Contact List

Fumio Matsuda
lab head
Fumio Matsuda
contact affiliation	Graduate School of Information Science a
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000167/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000167/