PXD004707

PXD004707 is an original dataset announced via ProteomeXchange.

Title	Quantitative Lipid Droplet Proteome Analysis Identifies Annexin A3 as a Cofactor for HCV Particle Production
Description	Cytosolic lipid droplets (LDs) are vital to Hepatitis C Virus (HCV) infection as the putative sites of virion assembly. To identify novel regulators of HCV particle production, we performed quantitative LD proteome analysis. Huh7.5 cells were labeled by stable isotope labeling with heavy amino acids in cell culture (SILAC) and subsequently infected with an HCV Jc1 reporter virus. After selection for HCV-infected cells, equal amounts of HCV-infected and uninfected control cells were mixed, LDs were isolated and analyzed by LC-ESI-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:31:43.398.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcus Wurlitzer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q-Tof Premier

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-03 02:43:49	ID requested
1	2017-10-24 01:42:58	announced
⏵ 2	2024-10-22 04:31:45	announced	2024-10-22: Updated project metadata.

R, ö, sch K, Kwiatkowski M, Hofmann S, Sch, ö, bel A, Gr, ü, ttner C, Wurlitzer M, Schl, ü, ter H, Herker E, Quantitative Lipid Droplet Proteome Analysis Identifies Annexin A3 as a Cofactor for HCV Particle Production. Cell Rep, 16(12):3219-3231(2016) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS, HCV, SILAC, quantitative proteomics,Lipid Droplets

Eva Herker
contact affiliation	Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
contact email	eva.herker@hpi.uni-hamburg.de
lab head
Marcus Wurlitzer
contact affiliation	Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
contact email	m.wurlitzer@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004707

PRIDE project URI

• PRIDE
  1. PXD004707
     1. Label: PRIDE project
     2. Name: Quantitative Lipid Droplet Proteome Analysis Identifies Annexin A3 as a Cofactor for HCV Particle Production