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PXD004706

PXD004706 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMSP22.8 is a protease inhibitor-like protein involved in shell mineralization in the edible mussel Mytilus galloprovincialis
DescriptionMSP22.8 is a shell matrix protein described in Mytilus galloprovincialis. During the characterization of the monoclonal antibody M22.8, we have demostrated that MSP22.8 is secreted into the extrapallial space by cells of the mantle edge epithelium. The protein is detected in the extrapallial fluid and extrapallial hemocytes and finally becomes part of the shell matrix framework. In order to isolate the protein from the extrapallial fluid, a multi-step purification strategy was designed, involving ammonium sulfate precipitation followed by affinity or size-exclusion chromatography. Eluted fractions were further processed by SDS-PAGE or 2D-PAGE. Positivity of fractions, bands and spots were checked by Western or Dot blot. Positive bands and spots were manually excised and sent for mass spectrometry experiments for protein identification.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:39:57.240.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJUAN CALVO
SpeciesList scientific name: Mytilus galloprovincialis (Mediterranean mussel); NCBI TaxID: 29158;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-08-03 02:25:37ID requested
12017-08-15 01:58:20announced
22017-10-09 02:25:55announcedUpdated publication reference for PubMed record(s): 28979842.
32024-10-22 04:39:58announced2024-10-22: Updated project metadata.
Publication List
Calvo-Iglesias J, P, é, rez-Est, é, vez D, Gonz, á, lez-Fern, á, ndez Á, . FEBS Open Bio, 7(10):1539-1556(2017) [pubmed]
10.1002/2211-5463.12286;
Keyword List
curator keyword: Biological
submitter keyword: LC-MSMS, extrapallial liquid, shell matrix protein,Mytilus galloprovincialis
Contact List
África González-Fernández
contact affiliationImmunology, Biomedical Research Center (CINBIO) and Institute of Biomedical Research of Vigo (IBIV), University of Vigo, Vigo, Spain
contact emailafrica@uvigo.es
lab head
JUAN CALVO
contact affiliationImmunology, Biomedical Research Center (CINBIO) and Institute of Biomedical Research of Vigo (IBIV), University of Vigo, Vigo, Spain
contact emailxoanci@hotmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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