PXD004706

PXD004706 is an original dataset announced via ProteomeXchange.

Title	MSP22.8 is a protease inhibitor-like protein involved in shell mineralization in the edible mussel Mytilus galloprovincialis
Description	MSP22.8 is a shell matrix protein described in Mytilus galloprovincialis. During the characterization of the monoclonal antibody M22.8, we have demostrated that MSP22.8 is secreted into the extrapallial space by cells of the mantle edge epithelium. The protein is detected in the extrapallial fluid and extrapallial hemocytes and finally becomes part of the shell matrix framework. In order to isolate the protein from the extrapallial fluid, a multi-step purification strategy was designed, involving ammonium sulfate precipitation followed by affinity or size-exclusion chromatography. Eluted fractions were further processed by SDS-PAGE or 2D-PAGE. Positivity of fractions, bands and spots were checked by Western or Dot blot. Positive bands and spots were manually excised and sent for mass spectrometry experiments for protein identification.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:57.240.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JUAN CALVO
SpeciesList	scientific name: Mytilus galloprovincialis (Mediterranean mussel); NCBI TaxID: 29158;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-03 02:25:37	ID requested
1	2017-08-15 01:58:20	announced
2	2017-10-09 02:25:55	announced	Updated publication reference for PubMed record(s): 28979842.
⏵ 3	2024-10-22 04:39:58	announced	2024-10-22: Updated project metadata.

Calvo-Iglesias J, P, é, rez-Est, é, vez D, Gonz, á, lez-Fern, á, ndez Á, . FEBS Open Bio, 7(10):1539-1556(2017) [pubmed]

10.1002/2211-5463.12286;

curator keyword: Biological

submitter keyword: LC-MSMS, extrapallial liquid, shell matrix protein,Mytilus galloprovincialis

África González-Fernández
contact affiliation	Immunology, Biomedical Research Center (CINBIO) and Institute of Biomedical Research of Vigo (IBIV), University of Vigo, Vigo, Spain
contact email	africa@uvigo.es
lab head
JUAN CALVO
contact affiliation	Immunology, Biomedical Research Center (CINBIO) and Institute of Biomedical Research of Vigo (IBIV), University of Vigo, Vigo, Spain
contact email	xoanci@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD004706

PRIDE project URI

• PRIDE
  1. PXD004706
     1. Label: PRIDE project
     2. Name: MSP22.8 is a protease inhibitor-like protein involved in shell mineralization in the edible mussel Mytilus galloprovincialis