PXD004706 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MSP22.8 is a protease inhibitor-like protein involved in shell mineralization in the edible mussel Mytilus galloprovincialis |
Description | MSP22.8 is a shell matrix protein described in Mytilus galloprovincialis. During the characterization of the monoclonal antibody M22.8, we have demostrated that MSP22.8 is secreted into the extrapallial space by cells of the mantle edge epithelium. The protein is detected in the extrapallial fluid and extrapallial hemocytes and finally becomes part of the shell matrix framework. In order to isolate the protein from the extrapallial fluid, a multi-step purification strategy was designed, involving ammonium sulfate precipitation followed by affinity or size-exclusion chromatography. Eluted fractions were further processed by SDS-PAGE or 2D-PAGE. Positivity of fractions, bands and spots were checked by Western or Dot blot. Positive bands and spots were manually excised and sent for mass spectrometry experiments for protein identification. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:39:57.240.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | JUAN CALVO |
SpeciesList | scientific name: Mytilus galloprovincialis (Mediterranean mussel); NCBI TaxID: 29158; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-08-03 02:25:37 | ID requested | |
1 | 2017-08-15 01:58:20 | announced | |
2 | 2017-10-09 02:25:55 | announced | Updated publication reference for PubMed record(s): 28979842. |
⏵ 3 | 2024-10-22 04:39:58 | announced | 2024-10-22: Updated project metadata. |
Publication List
Calvo-Iglesias J, P, é, rez-Est, é, vez D, Gonz, á, lez-Fern, á, ndez Á, . FEBS Open Bio, 7(10):1539-1556(2017) [pubmed] |
10.1002/2211-5463.12286; |
Keyword List
curator keyword: Biological |
submitter keyword: LC-MSMS, extrapallial liquid, shell matrix protein,Mytilus galloprovincialis |
Contact List
África González-Fernández |
contact affiliation | Immunology, Biomedical Research Center (CINBIO) and Institute of Biomedical Research of Vigo (IBIV), University of Vigo, Vigo, Spain |
contact email | africa@uvigo.es |
lab head | |
JUAN CALVO |
contact affiliation | Immunology, Biomedical Research Center (CINBIO) and Institute of Biomedical Research of Vigo (IBIV), University of Vigo, Vigo, Spain |
contact email | xoanci@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004706
- Label: PRIDE project
- Name: MSP22.8 is a protease inhibitor-like protein involved in shell mineralization in the edible mussel Mytilus galloprovincialis