PXD004698

PXD004698 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Zinc-responsive labeling in live cells for novel conditional proteomics
Description	Zinc signaling and dynamics play significant roles in many physiological responses and diseases. To understand the physiological roles of zinc in detail, comprehensive identification of proteins under high concentration of mobile zinc ion is crucial. In this report, we developed a novel “conditional proteomics” based on a chemical proteomic strategy with designer zinc-responsive labeling reagents (AIZin). In this conditional proteomics, a subset of proteins in a zinc-rich environment is tagged and identified in combination with quantitative mass spectrometry. This method was successfully applied to elucidate zinc dyshomeostasis induced by nitric oxide-triggered oxidative stress in glioma cells and unveiled dynamic changes of the zinc-related proteomes. Moreover, a subset of protein composition identified by our method allowed the characterization of unknown zinc-rich vesicles generated by oxidative stress as endoplasmic reticulum-Golgi-related vesicles.
HostingRepository	jPOST
AnnounceDate	2016-08-24
AnnouncementXML	Submission_2022-09-18_02:25:05.065.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004698
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hironori Achiwa
SpeciesList	scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; TMT6plex-126 reporter+balance reagent N6-acylated lysine; TMT6plex-126 reporter+balance reagent acylated N-terminal
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-01 18:25:18	ID requested
1	2016-08-24 03:15:03	announced
2	2017-03-31 00:07:18	announced	Updated DigitalObjectIdentifier.
3	2018-09-18 22:46:44	announced	Updated CV, FTP location and Modification list.
4	2021-02-04 17:03:59	announced	2021-02-04: Updated FTP location.
⏵ 5	2022-09-18 02:25:05	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: conditional proteomics, chemical proteomics, TMT

submitter keyword: conditional proteomics, chemical proteomics, TMT

Contact List

Itaru Hamachi
lab head
Hironori Achiwa
contact affiliation	Kyoto Univ.
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000159/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000159/