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PXD004698

PXD004698 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleZinc-responsive labeling in live cells for novel conditional proteomics
DescriptionZinc signaling and dynamics play significant roles in many physiological responses and diseases. To understand the physiological roles of zinc in detail, comprehensive identification of proteins under high concentration of mobile zinc ion is crucial. In this report, we developed a novel “conditional proteomics” based on a chemical proteomic strategy with designer zinc-responsive labeling reagents (AIZin). In this conditional proteomics, a subset of proteins in a zinc-rich environment is tagged and identified in combination with quantitative mass spectrometry. This method was successfully applied to elucidate zinc dyshomeostasis induced by nitric oxide-triggered oxidative stress in glioma cells and unveiled dynamic changes of the zinc-related proteomes. Moreover, a subset of protein composition identified by our method allowed the characterization of unknown zinc-rich vesicles generated by oxidative stress as endoplasmic reticulum-Golgi-related vesicles.
HostingRepositoryjPOST
AnnounceDate2016-08-24
AnnouncementXMLSubmission_2022-09-18_02:25:05.065.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD004698
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHironori Achiwa
SpeciesList scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; TMT6plex-126 reporter+balance reagent N6-acylated lysine; TMT6plex-126 reporter+balance reagent acylated N-terminal
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-08-01 18:25:18ID requested
12016-08-24 03:15:03announced
22017-03-31 00:07:18announcedUpdated DigitalObjectIdentifier.
32018-09-18 22:46:44announcedUpdated CV, FTP location and Modification list.
42021-02-04 17:03:59announced2021-02-04: Updated FTP location.
52022-09-18 02:25:05announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: conditional proteomics, chemical proteomics, TMT
Contact List
Itaru Hamachi
lab head
Hironori Achiwa
contact affiliationKyoto Univ.
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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