PXD004695

PXD004695 is an original dataset announced via ProteomeXchange.

Title	Saprolegnia parasitica proteome
Description	In this project, we made a comprehensive quantitative proteomic analysis of four defined developmental stages of S. parasitica (mycelium, primary cysts, secondary cysts and germinated cysts) to gain greater insight into the types of proteins specifically linked to each stage. A total of 2423 unique proteins were identified using qualitative (gel-based) and quantitative (iTRAQ, isobaric tags for relative and absolute quantitation) approaches. Of these, 358 proteins, associated with various biological processes, were found to be significantly enriched between different life cycle stages of S. parasitica. The transcript abundance of several cyst and mycelium enriched proteins was also checked by quantitative real-time PCR. This is the first large scale proteomic analysis on Saprolegnia and the data from this study will enhance our current knowledge about this pathogen by identifying biological processes key to each developmental stage.
HostingRepository	PRIDE
AnnounceDate	2018-08-10
AnnouncementXML	Submission_2019-02-12_08:11:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vaibhav Srivastava
SpeciesList	scientific name: Oomycetes; NCBI TaxID: 4762;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	Xevo Q-Tof

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-01 07:46:55	ID requested
1	2018-08-10 07:06:51	announced
⏵ 2	2019-02-12 08:11:14	announced	Updated publication reference for PubMed record(s): 29375523.

Srivastava V, Rezinciuc S, Bulone V, . Front Microbiol, 8():2658(2017) [pubmed]

curator keyword: Biological

submitter keyword: Saprolegnia parasitica, primary cysts, secondary cysts, germinated cyst, mycelium, mass spectrometry, oomycete, quantitative proteomics

Vincent Bulone / Vaibhav Srivastava
contact affiliation	Division of Glycoscience, Royal Institute of Technology (KTH), AlbaNova University Centre, Stockholm, SE-10691, Sweden;
contact email	bulone@kth.se
lab head
Vaibhav Srivastava
contact affiliation	Biotechnology
contact email	vasri@kth.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD004695

PRIDE project URI

• PRIDE
  1. PXD004695
     1. Label: PRIDE project
     2. Name: Saprolegnia parasitica proteome