PXD004690

PXD004690 is an original dataset announced via ProteomeXchange.

Title	Hydrophobic tagging-assisted N-termini enrichment (HYTANE) for in-depth N-terminome analysis
Description	The analysis of protein N-termini is of great importance for understanding the protein function and elucidating the proteolytic processing. Herein, we develop a negative enrichment strategy, termed as hydrophobic tagging-assisted N-termini enrichment (HYTANE) to achieve a global N-terminome analysis. The HYTANE strategy showed a high efficiency in hydrophobic tagging and C18 material-assisted depletion using bovine serum albumin (BSA) as the sample. Furthermore, this strategy was also applied to N-termini profiling from S. cerevisiaecell lysates and enabled the identification of 1096 protein N-termini, representing the largest N-terminome dataset of S. cerevisiae. The identified N-terminal peptides accounted for 99% of all identified peptides and no deficiency in acidic and histidine-containing N-terminal peptides was observed. The presented HYTANE strategy is therefore a highly selective, efficient and unbiased strategy for the large scale N-terminome analysis.
HostingRepository	PRIDE
AnnounceDate	2016-08-19
AnnouncementXML	Submission_2016-08-19_00:47:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lingfan Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-01 03:14:21	ID requested
⏵ 1	2016-08-19 00:47:18	announced

Chen L, Shan Y, Weng Y, Sui Z, Zhang X, Liang Z, Zhang L, Zhang Y, Hydrophobic Tagging-Assisted N-Termini Enrichment for In-Depth N-Terminome Analysis. Anal Chem, 88(17):8390-5(2016) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Sacchoromyces cerevisiae,Jurkat,n-termini,caspase

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lihuazhang@dicp.ac.cn
lab head
Lingfan Chen
contact affiliation	Dalian Institute of Chemical Physics
contact email	anxuanmuma@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004690
     1. Label: PRIDE project
     2. Name: Hydrophobic tagging-assisted N-termini enrichment (HYTANE) for in-depth N-terminome analysis