PXD004686

PXD004686 is an original dataset announced via ProteomeXchange.

Title	Novel insight into the composition of human single-stranded DNA-binding protein 1 (hSSB1)-containing protein complexes
Description	Single-stranded DNA-binding proteins are essential cellular components required for the protection, metabolism and processing of single-stranded DNA. Human single-stranded DNA-binding protein 1 (hSSB1) is one such protein, with described roles in genome stability maintenance and transcriptional regulation. As yet, however, the mechanisms through which hSSB1 functions and the binding partners with which it interacts remain poorly understood. In this work, hSSB1 was immunoprecipitated from cell lysate samples that had been enriched for non-soluble nuclear proteins and hSSB1-associating proteins identified by mass spectrometry. In doing so, 334 potential hSSB1-associating proteins were identified, with known roles in a range of distinct biological processes. Unexpectedly, whilst hSSB1 has largely been studied in a genome stability context, few other DNA repair or replication proteins were detected. By contrast, a large number of proteins were identified with roles in mRNA metabolism, reflecting a currently emerging area of hSSB1 study. In addition, numerous proteins were detected that comprise various chromatin-remodelling complexes. These findings provide new insight into the binding partners of hSSB1 and will likely function as a platform for future research.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:12:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dorothy Loo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	6520 Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-01 01:36:14	ID requested
1	2016-12-22 01:07:38	announced
⏵ 2	2017-10-24 03:12:34	announced	Updated project metadata.

Ashton NW, Loo D, Paquet N, O'Byrne KJ, Richard DJ, Novel insight into the composition of human single-stranded DNA-binding protein 1 (hSSB1)-containing protein complexes. BMC Mol Biol, 17(1):24(2016) [pubmed]

curator keyword: Biological

submitter keyword: hSSB1, NABP2, chromatin remodelling, mRNA metabolism, INTS3

Derek John Richard
contact affiliation	School of Biomedical Research, Institute of Health and Biomedical Innovation at the Translational Research Institute, 37 Kent Street, Woolloongabba, QLD 4102, Australia
contact email	derek.richard@qut.edu.au
lab head
Dorothy Loo
contact affiliation	Hill Laboratory
contact email	d.loo@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004686

PRIDE project URI

• PRIDE
  1. PXD004686
     1. Label: PRIDE project
     2. Name: Novel insight into the composition of human single-stranded DNA-binding protein 1 (hSSB1)-containing protein complexes