PXD004676

PXD004676 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cellular ADP-ribosylome characterization with HCD and EThcD
Description	Protein ADP-ribosylation is a post-translational modification involved in important physiological and pathological processes. Here, we present an optimized method using the Orbitrap Fusion Tribrid mass spectrometer, which drastically improves the overall ADP-ribosylation site localization score and boosts the identification of ADP-ribosylated peptides. We compared the HCD, ETcaD or EThcD fragmentation methods for the identification of ADP-ribose acceptor sites in a complex cellular sample and further combined them in a product dependent manner, exploiting the unique fragmentation properties of the ADP-ribose moiety as a trigger for a targeted fragmentation of modified peptides.
HostingRepository	MassIVE
AnnounceDate	2018-02-23
AnnouncementXML	Submission_2018-02-23_07:05:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vera
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-28 06:38:10	ID requested
1	2017-01-04 14:15:33	announced
⏵ 2	2018-02-23 07:05:18	announced	Making dataset [MSV000080008] public

Publication List

Bilan V, Leutert M, Nanni P, Panse C, Hottiger MO, Combining Higher-Energy Collision Dissociation and Electron-Transfer/Higher-Energy Collision Dissociation Fragmentation in a Product-Dependent Manner Confidently Assigns Proteomewide ADP-Ribose Acceptor Sites. Anal Chem, 89(3):1523-1530(2017) [pubmed]

Bilan V, Leutert M, Nanni P, Panse C, Hottiger MO, Combining Higher-Energy Collision Dissociation and Electron-Transfer/Higher-Energy Collision Dissociation Fragmentation in a Product-Dependent Manner Confidently Assigns Proteomewide ADP-Ribose Acceptor Sites. Anal Chem, 89(3):1523-1530(2017) [pubmed]

Keyword List

submitter keyword: ADP-ribosylome, EThcD, HCD, Product dependent method

submitter keyword: ADP-ribosylome, EThcD, HCD, Product dependent method

Contact List

Prof. Michael O.Hottiger
lab head
Vera
contact affiliation	Universitz of Zurich
contact email	vera.bilan@dmmd.uzh.ch
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080008

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080008