PXD004670

PXD004670 is an original dataset announced via ProteomeXchange.

Title	Insight into enzymatic degradation of corn, wheat, and soybean cell wall cellulose using quantitative secretome analysis of Aspergillus fumigatus
Description	Aspergillus fumigatus, a saprophytic filamentous fungus, is known to harbor 263 glycoside hydrolase (GH) encoding genes, suggesting that A. fumigatus is an efficient lignocellulose degrader. Hence the present study uses corn, wheat or soybean as a sole carbon source to culture A. fumigatus under animal physiological condition as to understand how cellulolytic enzymes work together to achieve an efficient degradation of lignocellulose. Using isobaric tags for relative and absolute quantification (iTRAQ) approach, total of ~600 extracellular proteins were identified and quantified, in which ~50 proteins were involved in lignocellulolysis, including cellulases, hemicellulases, lignin-degrading enzymes and some hypothetical proteins.Functional classification of identified proteins showed 15% cellulases, 14 % hydrolases acting on glycosyl bond, and 22% hemicellulases。The approach of highly sensitive quantitative analysis of proteome and transcriptome respond to the probable enzyme combination required in agro industries and animal feeding purpose towards more efficient cellulose degradation and therefore better digestion of intracellular starch. Furthermore, number of important GH family protein, some CBM and polysaccharide lyase family proteins have been identified, which could attribute towards better degradation of lignocelluloses to release starch.
HostingRepository	PRIDE
AnnounceDate	2016-09-29
AnnouncementXML	Submission_2016-09-29_12:23:08.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004670
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Prakriti Sharma
SpeciesList	scientific name: Aspergillus fumigatus Af293; NCBI TaxID: 330879;
ModificationList	Methylthio; Oxidation; iTRAQ8plex; Acetyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-28 02:37:55	ID requested
⏵ 1	2016-09-29 12:23:09	announced

Sharma Ghimire P, Ouyang H, Wang Q, Luo Y, Shi B, Yang J, L, ü Y, Jin C, Insight into Enzymatic Degradation of Corn, Wheat, and Soybean Cell Wall Cellulose Using Quantitative Secretome Analysis of Aspergillus fumigatus. J Proteome Res, 15(12):4387-4402(2016) [pubmed]

curator keyword: Biological

submitter keyword: Aspergillus fumigatus, lignocelluloses, iTRAQ，secretome, lignocellulolytic proteins, forage

Cheng Jin
contact affiliation	State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
contact email	jinc@im.ac.cn
lab head
Prakriti Sharma
contact affiliation	Chinese Academy of Sciences
contact email	sharprakriti@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004670
     1. Label: PRIDE project
     2. Name: Insight into enzymatic degradation of corn, wheat, and soybean cell wall cellulose using quantitative secretome analysis of Aspergillus fumigatus