PXD004669

PXD004669 is an original dataset announced via ProteomeXchange.

Title	Human KAT2A/B-acetylome MudPIT
Description	Comprehensive identification of acetylated proteins targeted by the lysine acetyltransferases KAT2A (GCN5) and KAT2B (PCAF), in human cells. KAT2A/B-dependent acetylated proteins were identified by comparing acetylated proteins in whole cell extract of control cells and cells in which KAT2A and KAT2B were simultaneously knocked down. The identification of acetylated proteins was performed using an enrichment-free approach. In order to do so, while facing the challenge of identifying low abundant acetylated peptides within a complex sample mixture, we have screened acetylated peptides starting from a large amount of material, equivalent to 50ug of protein digest (maximum loading capacity of our analytical chromatographic column). By analysing such a large amount of peptides, the level of acetylated peptides was then increased and thus acetylated peptides became easily detectable by mass spectrometry, without the need for enrichment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:31:50.964.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mathilde Joint
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-28 02:19:31	ID requested
1	2016-12-12 13:54:56	announced
2	2017-10-24 02:19:59	announced	Updated project metadata.
⏵ 3	2024-10-22 04:31:51	announced	2024-10-22: Updated project metadata.

10.1038/ncomms13227;

Fournier M, Orpinell M, Grauffel C, Scheer E, Garnier JM, Ye T, Chavant V, Joint M, Esashi F, Dejaegere A, G, ö, nczy P, Tora L, KAT2A/KAT2B-targeted acetylome reveals a role for PLK4 acetylation in preventing centrosome amplification. Nat Commun, 7():13227(2016) [pubmed]

curator keyword: Biological

submitter keyword: acetylation, non-histone targets, KAT2A(GCN5), centrosome duplication, KAT2B (PCAF), PLK4

Laszlo TORA
contact affiliation	Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)- UMR 7104 CNRS, INSERM U964, Université de Strasbourg (UdS)
contact email	laszlo@igbmc.fr
lab head
Mathilde Joint
contact affiliation	Proteomics plateform IGBMC - CNRS UMR 7104 - Inserm U 964
contact email	mathilde.joint@igbmc.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004669

PRIDE project URI

• PRIDE
  1. PXD004669
     1. Label: PRIDE project
     2. Name: Human KAT2A/B-acetylome MudPIT