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PXD004659

PXD004659 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePAXX is an accessory c-NHEJ factor that associates with Ku70 and has overlapping functions with XLF
DescriptionIn mammalian cells, classical non-homologous end joining (c-NHEJ) is critical for DNA double-strand break repair induced by ionizing radiation and during V(D)J recombination in developing B and T lymphocytes. Recently, PAXX was identified as a new c-NHEJ core component. We report here, that PAXX-deficient cells exhibit a cellular phenotype uncharacteristic of a deficiency in c-NHEJ core components. PAXX-deficient cells display normal sensitivity to radiomimetic drugs, are proficient in transient V(D)J recombination assays, and do not shift towards higher micro-homology usage in plasmid repair assays. Although PAXX-deficient cells lack c-NHEJ phenotypes, PAXX forms a ternary complex with Ku bound to DNA. Formation of this complex involves an interaction with Ku70 and requires a bare DNA extension for stability. Moreover, the relatively weak Ku-dependent stimulation of LIG4/XRCC4 activity by PAXX is unmasked by XLF ablation. Thus, PAXX plays an accessory role during c-NHEJ that is largely overlapping with XLF’s function.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:32:47.654.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAUDEBERT Stephane
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-07-27 01:17:00ID requested
12016-10-06 06:08:21announced
22024-10-22 04:32:49announced2024-10-22: Updated project metadata.
Publication List
Tadi SK, Tellier-Leb, è, gue C, Nemoz C, Drevet P, Audebert S, Roy S, Meek K, Charbonnier JB, Modesti M, PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF. Cell Rep, 17(2):541-555(2016) [pubmed]
10.1016/j.celrep.2016.09.026;
Keyword List
curator keyword: Biomedical
submitter keyword: Non-homologous end joining, micro-homology mediated end joining, V(D)J recombination,Genomic instability, DNA double-strand break
Contact List
Borg Jean-Paul
contact affiliationCRCM: Aix Marseille Univ, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France
contact emailjean-paul.borg@inserm.fr
lab head
AUDEBERT Stephane
contact affiliationMarseille Proteomic, Centre de Recherche en Cancérologie de Marseille, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes, 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France
contact emailstephane.audebert@inserm.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
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