PXD004659 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PAXX is an accessory c-NHEJ factor that associates with Ku70 and has overlapping functions with XLF |
Description | In mammalian cells, classical non-homologous end joining (c-NHEJ) is critical for DNA double-strand break repair induced by ionizing radiation and during V(D)J recombination in developing B and T lymphocytes. Recently, PAXX was identified as a new c-NHEJ core component. We report here, that PAXX-deficient cells exhibit a cellular phenotype uncharacteristic of a deficiency in c-NHEJ core components. PAXX-deficient cells display normal sensitivity to radiomimetic drugs, are proficient in transient V(D)J recombination assays, and do not shift towards higher micro-homology usage in plasmid repair assays. Although PAXX-deficient cells lack c-NHEJ phenotypes, PAXX forms a ternary complex with Ku bound to DNA. Formation of this complex involves an interaction with Ku70 and requires a bare DNA extension for stability. Moreover, the relatively weak Ku-dependent stimulation of LIG4/XRCC4 activity by PAXX is unmasked by XLF ablation. Thus, PAXX plays an accessory role during c-NHEJ that is largely overlapping with XLF’s function. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:32:47.654.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AUDEBERT Stephane |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-27 01:17:00 | ID requested | |
1 | 2016-10-06 06:08:21 | announced | |
⏵ 2 | 2024-10-22 04:32:49 | announced | 2024-10-22: Updated project metadata. |
Publication List
Tadi SK, Tellier-Leb, è, gue C, Nemoz C, Drevet P, Audebert S, Roy S, Meek K, Charbonnier JB, Modesti M, PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF. Cell Rep, 17(2):541-555(2016) [pubmed] |
10.1016/j.celrep.2016.09.026; |
Keyword List
curator keyword: Biomedical |
submitter keyword: Non-homologous end joining, micro-homology mediated end joining, V(D)J recombination,Genomic instability, DNA double-strand break |
Contact List
Borg Jean-Paul |
contact affiliation | CRCM: Aix Marseille Univ, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France |
contact email | jean-paul.borg@inserm.fr |
lab head | |
AUDEBERT Stephane |
contact affiliation | Marseille Proteomic, Centre de Recherche en Cancérologie de Marseille, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes, 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France |
contact email | stephane.audebert@inserm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004659
- Label: PRIDE project
- Name: PAXX is an accessory c-NHEJ factor that associates with Ku70 and has overlapping functions with XLF