PXD004655

PXD004655 is an original dataset announced via ProteomeXchange.

Title	SILAC-Tp (12 importins)
Description	Importin-beta family proteins are nucleocytoplasmic transport receptors (NTRs), and they transport most nuclear proteins as their cargoes into and out of the nuclei. Human cells have 20 species of importin-beta family NTRs, of which 10 (importin-beta, transportin-1, -2, -SR, importin-4, -5, -7, -8, -9, and -11) are nuclear import receptors and 2 (importin-13, and exportin-4) are bi-directional receptors. Here, we identified the import cargo proteins of these 12 NTRs by a method called SILAC-Tp, which employs stable isotope labeling with amino acids in cell culture (SILAC), an in vitro reconstituted transport system, and LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2017-01-20
AnnouncementXML	Submission_2017-02-02_07:19:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Makoto Kimura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-25 23:49:16	ID requested
1	2017-01-20 05:03:01	announced
⏵ 2	2017-02-02 07:19:58	announced	Updated publication reference for PubMed record(s): 28117667.

Kimura M, Morinaka Y, Imai K, Kose S, Horton P, Imamoto N, Extensive cargo identification reveals distinct biological roles of the 12 importin pathways. Elife, 6():(2017) [pubmed]

curator keyword: Biological

submitter keyword: human, importin, transportin, cargo, nuclear transport, SILAC, LC-MS/MS

Naoko Imamoto
contact affiliation	Cellular Dynamics Laboratory, RIKEN, Japan
contact email	nimamoto@riken.jp
lab head
Makoto Kimura
contact affiliation	RIKEN
contact email	makimura@riken.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD004655

PRIDE project URI

• PRIDE
  1. PXD004655
     1. Label: PRIDE project
     2. Name: SILAC-Tp (12 importins)