PXD004654

PXD004654 is an original dataset announced via ProteomeXchange.

Title	Temperature-dependent regulation of the Ochrobactrum anthropi proteome
Description	Ochrobactrum anthropi is a gram-negative rod belonging to the Brucellaceae family, able to colonize a variety of environments, and actually reported as a human opportunistic pathogen. Despite its low virulence, the bacterium causes a growing number of hospital-acquired infections mainly, but not exclusively, in immunocompromised patients. The aim of this study was to obtain an overview of the global proteome changes occurring in O. anthropi in response to different growth temperatures, in order to achieve a major understanding of the mechanisms by which the bacterium adapts to different habitats and to identify some potential virulence factors. Combined quantitative mass spectrometry-based proteomics and bioinformatics approaches were carried out on two O. anthropi strains grown at temperatures miming soil/plants habitat (25 C) and human host environment (37 C), respectively. Proteomic analysis led to the identification of over 150 differentially expressed proteins in both strains, out of over 1200 total protein identifications. Among them, proteins responsible for heat shock response (DnaK, GrpE), motility (FliC, FlgG, FlgE), and putative virulence factors (TolB) were identified. The study represents the first quantitative proteomic analysis of O. anthropi performed by high-resolution quantitative mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_02:40:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariaconcetta Varano
SpeciesList	scientific name: Ochrobactrum anthropi ATCC 49188; NCBI TaxID: 439375;
ModificationList	4x(2)H labeled dimethylated L-lysine; monohydroxylated residue; 4x(2)H labeled alpha-dimethylamino N-terminal residue; N6: N6-dimethyl-L-lysine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-25 06:04:40	ID requested
1	2016-12-13 06:12:29	announced
⏵ 2	2017-10-24 02:40:56	announced	Updated project metadata.

Varano M, Gaspari M, Quirino A, Cuda G, Liberto MC, Foc, à A, Temperature-dependent regulation of the Ochrobactrum anthropi proteome. Proteomics, 16(23):3019-3024(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Bacteria / Heat adaptation / Ochrobactrum anthropi / Quantitative proteomics / Stable isotope dimethyl labeling

Marco Gaspari
contact affiliation	Department of Experimental & Clinical Medicine Building G, Level 3 “Magna Græcia” University of Catanzaro Campus “S. Venuta”, Viale Europa, Loc. Germaneto 88100 Catanzaro- Italy
contact email	gaspari@unicz.it
lab head
Mariaconcetta Varano
contact affiliation	University of Catanzaro
contact email	mary.varano1@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004654
     1. Label: PRIDE project
     2. Name: Temperature-dependent regulation of the Ochrobactrum anthropi proteome