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PXD004650

PXD004650 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSite-specific K63 ubiquitinomics reveals stabilization of ribosomes in response to oxidative stress
DescriptionReprograming of protein synthesis is an essential cellular process to tolerate and resist to stressing conditions. A variety of mechanisms are known to regulate translation at initiation but cells can also control proteins synthesis after the initiation checkpoint. We previously showed that K63 ubiquitin can modify ribosome proteins in response to oxidative stress. However, the mechanism by how K63 ubiquitin impacts ribosome function is entirely unknown. Here we characterized > 1000 K63 ubiquitin sites in the yeast Saccharomyces cerevisiae by mass spectrometry, and showed that many sites clustered at the head of the 40S subunit in response to H2O2. Moreover, ribosomes lacking K63 ubiquitin were depleted in proteins from the translation initiation factor eIF3, particularly Tif35 (eIF3g), which impacted ribosome stability, and protein production. Our results provided new insights on the role of K63 ubiquitin in regulating the resistance to oxidative stress via a post-initiation control of translation.
HostingRepositoryPRIDE
AnnounceDate2018-12-06
AnnouncementXMLSubmission_2018-12-06_03:27:32.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGustavo Silva
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList6x(13)C: 2x(15)N labeled L-lysine; iodoacetamide derivatized residue
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-07-25 02:57:41ID requested
12018-12-06 03:27:33announced
Publication List
Back S, Gorman AW, Vogel C, Silva GM, Site-Specific K63 Ubiquitinomics Provides Insights into Translation Regulation under Stress. J Proteome Res, 18(1):309-318(2019) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: K63 ubiquitin, ubiquitinomics, translation regulation, yeast, oxidative stress
Contact List
Christine Vogel
contact affiliationCenter for Genomics and Systems Biology Department of Biology New York University, USA
contact emailcvogel@nyu.edu
lab head
Gustavo Silva
contact affiliationNew York University
contact emailgu_msilva@yahoo.com.br
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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