PXD004650 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Site-specific K63 ubiquitinomics reveals stabilization of ribosomes in response to oxidative stress |
Description | Reprograming of protein synthesis is an essential cellular process to tolerate and resist to stressing conditions. A variety of mechanisms are known to regulate translation at initiation but cells can also control proteins synthesis after the initiation checkpoint. We previously showed that K63 ubiquitin can modify ribosome proteins in response to oxidative stress. However, the mechanism by how K63 ubiquitin impacts ribosome function is entirely unknown. Here we characterized > 1000 K63 ubiquitin sites in the yeast Saccharomyces cerevisiae by mass spectrometry, and showed that many sites clustered at the head of the 40S subunit in response to H2O2. Moreover, ribosomes lacking K63 ubiquitin were depleted in proteins from the translation initiation factor eIF3, particularly Tif35 (eIF3g), which impacted ribosome stability, and protein production. Our results provided new insights on the role of K63 ubiquitin in regulating the resistance to oxidative stress via a post-initiation control of translation. |
HostingRepository | PRIDE |
AnnounceDate | 2018-12-06 |
AnnouncementXML | Submission_2018-12-06_03:27:32.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gustavo Silva |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | 6x(13)C: 2x(15)N labeled L-lysine; iodoacetamide derivatized residue |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-25 02:57:41 | ID requested | |
⏵ 1 | 2018-12-06 03:27:33 | announced | |
Publication List
Back S, Gorman AW, Vogel C, Silva GM, Site-Specific K63 Ubiquitinomics Provides Insights into Translation Regulation under Stress. J Proteome Res, 18(1):309-318(2019) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: K63 ubiquitin, ubiquitinomics, translation regulation, yeast, oxidative stress |
Contact List
Christine Vogel |
contact affiliation | Center for Genomics and Systems Biology Department of Biology New York University, USA |
contact email | cvogel@nyu.edu |
lab head | |
Gustavo Silva |
contact affiliation | New York University |
contact email | gu_msilva@yahoo.com.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD004650 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004650
- Label: PRIDE project
- Name: Site-specific K63 ubiquitinomics reveals stabilization of ribosomes in response to oxidative stress